A new bioactive glass with extremely high crystallization temperature and outstanding biological performance.

In this work, a new bioactive glass was designed, prepared by means of a melt-quenching route and characterized in terms of both thermal properties and biological performance. The main objective was to obtain a novel product with high temperature of crystallization in view of possible thermal treatments, as well as remarkable biological responsiveness. Thermal behavior was investigated by heating microscopy, differential thermal analysis (DTA) and sintering tests. The glass displayed a very high crystallization temperature and the samples remained completely amorphous after sintering. Bioactivity was evaluated by means of Simulated Body Fluid (SBF) assay, which is a widely used method to preliminary investigate samples' reactivity in vitro; the glass showed a strong apatite forming ability. Additionally, in order to exclude cytotoxic effects, biocompatibility was verified according to ISO standard 10993. Finally, the biological potential of the new glass was tested by using an innovative 3D cellular model, that mimicked the potential clinical application of a given biomaterial. Human bone marrow mesenchymal stem cells (BM-MSCs) were employed to study the performance of bioactive glass granules in such 3D cellular model. The results showed that the bioactive glass supported human BM-MSCs adhesion, colonization and bone differentiation. Thus, this new bioactive glass looks particularly promising for orthopedic applications, bone tissue engineering and regenerative medicine, especially when a thermal treatment is necessary for the production of specific devices.