Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, College of Chemistry and Material Science, Fujian Normal University, Fuzhou, Fujian 350007, China.
Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, College of Chemistry and Material Science, Fujian Normal University, Fuzhou, Fujian 350007, China.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110655. doi: 10.1016/j.msec.2020.110655. Epub 2020 Jan 8.
EPLA/nHAp composite microsphere, a novel drug delivery system potentially useful for the local delivery of alendronate (AL) to bone tissue was developed via the biomimetic mineralized deposition of nano-hydroxyapatite (nHAp) crystals on the surface of aminated modified polylactic acid (EPLA) microspheres. Scanning electron microscopy (SEM) observation showed that this system consisted of a polymer core with nanofiber network structure and inorganic coating composed of countless rod-like nanocrystalline particles, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD) confirmed that these particles were nHAp crystals. An efficient AL-loading can be realized by facile impregnation-adsorption method under suitable conditions due to the high adsorption capacity of EPLA/nHAp composite microspheres. The drug loading efficiency of microspheres was detected by indirect ultraviolet spectrophotometry. It was found that the adsorption capacity of EPLA/nHAp composite microsphere towards AL was increased nearly 5-fold compared with that of bare EPLA microspheres owing to the strong interaction between alendronate and hydroxyapatite. Meanwhile, in vitro release study showed that AL-loaded EPLA/nHAp microspheres had a more sustained drug release than AL-loaded EPLA microspheres, all these results demonstrated that the as-prepared EPLA/nHAp composite microsphere is an efficient carrier for the delivery and sustained release of AL. Furthermore, an in vitro cell culture study revealed that these composite microspheres presented a good biocompatibility, showing great potential for the applications in the biomedical field.
EPLA/nHAp 复合微球是一种新型药物传递系统，通过纳米羟基磷灰石 (nHAp) 晶体在氨基化改性聚乳酸 (EPLA) 微球表面的仿生矿化沉积，可将阿仑膦酸钠 (AL) 局部递送到骨组织。扫描电子显微镜 (SEM) 观察表明，该系统由聚合物核和纳米纤维网络结构组成，无机涂层由无数棒状纳米晶颗粒组成，傅里叶变换红外光谱 (FTIR) 和 X 射线衍射分析 (XRD) 证实这些颗粒为 nHAp 晶体。在适当的条件下，通过简单的浸渍-吸附法可以实现高效的 AL 负载，这是由于 EPLA/nHAp 复合微球具有高吸附能力。通过间接紫外分光光度法检测微球的载药量。结果发现，与裸 EPLA 微球相比，EPLA/nHAp 复合微球对 AL 的吸附容量增加了近 5 倍，这是由于 AL 与羟基磷灰石之间的强相互作用。同时，体外释放研究表明，负载 AL 的 EPLA/nHAp 微球的药物释放更持久，所有这些结果表明，所制备的 EPLA/nHAp 复合微球是 AL 传递和持续释放的有效载体。此外，体外细胞培养研究表明，这些复合微球具有良好的生物相容性，在生物医学领域具有广阔的应用前景。
