Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
Department of Natural Sciences, Faculty of Science and Engineering, University of Chester, Thornton Science Park, Pool Lane, Ince, Chester CH2 4NU, UK.
Biosensors (Basel). 2020 Mar 19;10(3):27. doi: 10.3390/bios10030027.
We demonstrate a facile methodology for the mass production of graphene oxide (GO) bulk-modified screen-printed electrodes (GO-SPEs) that are economical, highly reproducible and provide analytically useful outputs. Through fabricating GO-SPEs with varying percentage mass incorporations (2.5%, 5%, 7.5% and 10%) of GO, an electrocatalytic effect towards the chosen electroanalytical probes is observed, which increases with greater GO incorporated compared to bare/graphite SPEs. The optimum mass ratio of 10% GO to 90% carbon ink produces an electroanalytical signal towards dopamine (DA) and uric acid (UA) which is ca. ×10 greater in magnitude than that achievable at a bare/unmodified graphite SPE. Furthermore, 10% GO-SPEs exhibit a competitively low limit of detection (3σ) towards DA at ca. 81 nM, which is superior to that of a bare/unmodified graphite SPE at ca. 780 nM. The improved analytical response is attributed to the large number of oxygenated species inhabiting the edge and defect sites of the GO nanosheets, which are able to exhibit electrocatalytic responses towards inner-sphere electrochemical analytes. Our reported methodology is simple, scalable, and cost effective for the fabrication of GO-SPEs that display highly competitive LODs and are of significant interest for use in commercial and medicinal applications.
我们展示了一种简便的方法来大规模生产氧化石墨烯（GO）整体修饰的丝网印刷电极（GO-SPE），这种方法经济、高度重现且提供有用的分析输出。通过制造具有不同质量分数（2.5%、5%、7.5%和 10%）GO 的 GO-SPE，观察到对所选电分析探针的电催化效应，与裸/石墨 SPE 相比，随着更多 GO 的掺入而增加。最佳质量比为 10% GO 对 90%碳墨的电分析信号对多巴胺（DA）和尿酸（UA）的响应约为裸/未修饰石墨 SPE 的 10 倍。此外，10% GO-SPE 对 DA 的竞争检测限（3σ）约为 81 nM，优于裸/未修饰石墨 SPE 的约 780 nM。改进的分析响应归因于大量含氧物种栖息在 GO 纳米片的边缘和缺陷位，这些物种能够对内球电化学分析物表现出电催化响应。我们报道的方法简单、可扩展且具有成本效益，可用于制造显示出高竞争力检测限的 GO-SPE，这对商业和医疗应用具有重要意义。
