Lee Hun, Kim Hong Kyung, Shin Hae Sol, Han Soo Jung, Yoon Sangchul, Seong Je Kyung, Seo Kyoung Yul
1Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
2Korea Mouse Sensory Phenotyping Center, Yonsei University College of Medicine, Seoul, South Korea.
Lab Anim Res. 2020 Jan 7;36:1. doi: 10.1186/s42826-019-0031-z. eCollection 2020.
To investigate conditions that cause temporal lens opacity, we tested chemical and physical factors, such as anaesthesia dose, ocular surface dryness, and infrared (IR) light exposure in anaesthetised C57BL/6 N mice. Mice were anaesthetised with a low (80%; tiletamine/zolazepam 32 mg/kg and xylazine 8 mg/kg, intraperitoneal injection) or high (120%; 48 mg/kg and 12 mg/kg) dose of anaesthetic and examined every 5 min from 10 to 30 min after anaesthesia was induced. Lens opacity levels were assessed and graded (1-6) using the standard classification system. Regardless of the anaesthetic dose, lens opacity grade was 1-2 in moisturised eyes with application of 0.5% carboxymethylcellulose, and 5-6 in dry ocular surface conditions. Lens opacity in mice with high-dose anaesthetic in the dry ocular surface condition was not different from that of mice with low-dose anaesthetic. Lens opacity grade 1-2 was noted in eyes in the wet ocular surface condition, regardless of IR light exposure. During IR light exposure in eyes in the dry ocular surface condition, lens opacity (grade 6) in mice with high-dose anaesthetic was not different from that (grade 6) in mice with low-dose anaesthetic. We demonstrated that ocular surface dryness might be a relevant factor for the formation and progression of lens opacity in anesthetized C57BL/6 N mice. Anaesthesia dose and IR light exposure did not strongly influence lens opacity formation. Furthermore, eyes with corneal dryness-induced lens opacity recovered to normal status without additional intervention.
为了研究导致晶状体混浊的条件，我们在麻醉的C57BL/6 N小鼠中测试了化学和物理因素，如麻醉剂量、眼表干燥和红外（IR）光照射。小鼠分别用低剂量（80%；替来他明/唑拉西泮32 mg/kg和赛拉嗪8 mg/kg，腹腔注射）或高剂量（120%；48 mg/kg和12 mg/kg）的麻醉剂进行麻醉，并在诱导麻醉后10至30分钟内每隔5分钟进行检查。使用标准分类系统评估晶状体混浊程度并分级（1-6级）。无论麻醉剂量如何，在应用0.5%羧甲基纤维素的湿润眼睛中，晶状体混浊分级为1-2级，而在眼表干燥的情况下为5-6级。在眼表干燥条件下接受高剂量麻醉的小鼠的晶状体混浊与接受低剂量麻醉的小鼠没有差异。在湿润眼表条件下的眼睛中，无论是否暴露于红外光，均观察到晶状体混浊分级为1-2级。在眼表干燥条件下的眼睛接受红外光照射期间，高剂量麻醉小鼠的晶状体混浊（6级）与低剂量麻醉小鼠的（6级）没有差异。我们证明，眼表干燥可能是麻醉的C57BL/6 N小鼠晶状体混浊形成和进展的一个相关因素。麻醉剂量和红外光照射对晶状体混浊的形成没有强烈影响。此外，因角膜干燥引起晶状体混浊的眼睛在没有额外干预的情况下恢复到正常状态。
