Çalışır Metin, Akpınar Aysun, Poyraz Ömer, Göze Fahrettin, Çınar Ziynet
Department of Periodontology, Faculty of Dentistry, Adıyaman University, Adıyaman, Turkey.
Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.
J Vet Dent. 2019 Dec;36(4):257-265. doi: 10.1177/0898756420910531.
The purpose of this study was to evaluate the biochemical, morphometric, and histopathological changes associated with experimental periodontitis in rats in response to local administration of humic acid. Thirty-eight Wistar rats were divided into 5 experimental groups: nonligated (NL) group, ligature-only (LO) group, and ligature + local administration of humic acid (20, 80, and 150 mg/kg body weight per day for 15 days, respectively; L-20, L-80, and L-150 groups). Changes in alveolar bone levels were clinically measured as the distance from the cementoenamel junction to the alveolar bone crest with a stereomicroscope. Tissues were histopathologically examined to assess the osteoclast numbers, osteoblastic activity, and inflammatory cell infiltration among the study groups. Enzyme-linked immunosorbent assay interleukin1β (IL-1β) and IL-10 levels in serum and gingival homogenates were evaluated. At the end of 15 days, the alveolar bone loss was significantly higher in the LO group compared to the NL, L-20, and L-150 groups ( < .05). The osteoclast number in the LO group was significantly higher than the NL, L-20, and L-150 groups ( < .05). Inflammatory cell infiltration was significantly higher in the LO and L-80 groups than the other groups ( < .05). The highest serum and gingival homogenate IL-10 levels were determined in the NL group ( < .05). The serum and gingival homogenate IL-1β levels in LO group were significantly higher than the NL, L-20, and L-150 groups ( < .05). Within the limits of this study, it can be suggested that humic acid, when administered locally at 20 and 80 mg/kg doses, may prevent alveolar bone loss in the rat model.
本研究的目的是评估大鼠实验性牙周炎在局部施用腐殖酸后的生化、形态计量学和组织病理学变化。38只Wistar大鼠被分为5个实验组：未结扎（NL）组、仅结扎（LO）组、结扎+局部施用腐殖酸（分别为每天20、80和150 mg/kg体重，持续15天；L-20、L-80和L-150组）。用体视显微镜临床测量牙槽骨水平的变化，即从牙骨质釉质界到牙槽嵴顶的距离。对组织进行组织病理学检查，以评估各研究组中的破骨细胞数量、成骨细胞活性和炎性细胞浸润情况。评估血清和牙龈匀浆中酶联免疫吸附测定白细胞介素1β（IL-1β）和IL-10水平。在15天结束时，LO组的牙槽骨丧失明显高于NL、L-20和L-150组（P<0.05）。LO组的破骨细胞数量明显高于NL、L-20和L-150组（P<0.05）。LO和L-80组的炎性细胞浸润明显高于其他组（P<0.05）。NL组的血清和牙龈匀浆IL-10水平最高（P<0.05）。LO组的血清和牙龈匀浆IL-1β水平明显高于NL、L-20和L-150组（P<0.05）。在本研究范围内，可以认为，以20和80 mg/kg剂量局部施用腐殖酸可能预防大鼠模型中的牙槽骨丧失。
