两种新的薯蓣科薯蓣属甾体生物碱

Two new pregnane alkaloids from Sieb. et Zucc.

机构信息

Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, Shaanxi Province Key Laboratory of New Drugs and Chinese, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China.

The College of Life Sciences, Northwest University, Xi'an, China.

出版信息

Nat Prod Res. 2021 Nov;35(21):3888-3894. doi: 10.1080/14786419.2020.1744143. Epub 2020 Mar 25.

DOI:10.1080/14786419.2020.1744143

PMID:32208773

Abstract

Two new pregnane alkaloids, (20)-20-cinnamoylamino-3-dimethylamino-5-en-pregnane () and (20)-20-cinnamoylamino-3-dimethylamino-pregnane (), and four known alkaloids (+)-(20)-20-(dimethylamino)-3-(3'-isopropyl)-lactam-5-pregn-2-en-4-one (), axillaridine A (), pachysamine M () and 20-dimethylamino-16-hydroxy-3-senecioylamino-pregn-5-ene () were obtained from the whole herb of Sieb. et Zucc. Their structures were determined by various spectral techniques and computed electronic circular dichroism (ECD) data. Compounds were tested for cytotoxicity against three human tumor cell lines and a human umbilical vein endothelial cell (HUVEC) line. Compound exhibited moderate cytotoxicity against MCF-7, U251 and A549 cells with IC values of 15.01 ± 0.47 μM, 20.13 ± 1.34 μM and 20.04 ± 1.16 μM, respectively; compounds showed weak cytotoxic activity against three tumor cells.

摘要

从 Sieb. et Zucc. 的全草中分离得到了两个新的孕烷生物碱，（20）-20-肉桂酰氨基-3-二甲基氨基-5-烯孕烷（()和（20）-20-肉桂酰氨基-3-二甲基氨基孕烷（()，以及四个已知的生物碱 (+)-(20)-20-（二甲基氨基）-3-（3'-异丙基）-内酰胺-5-孕-2-烯-4-酮（()、axillaridine A ()、pachysamine M ()和 20-二甲基氨基-16-羟基-3-千里光酰氨基孕-5-烯（()。通过各种光谱技术和计算电子圆二色性（ECD）数据确定了它们的结构。对化合物 ()进行了对三种人肿瘤细胞系和人脐静脉内皮细胞（HUVEC）系的细胞毒性测试。化合物 ()对 MCF-7、U251 和 A549 细胞表现出中等的细胞毒性，IC 值分别为 15.01 ± 0.47 μM、20.13 ± 1.34 μM 和 20.04 ± 1.16 μM；化合物 ()对三种肿瘤细胞表现出较弱的细胞毒性活性。