Funayama S, Noshita T, Shinoda K, Haga N, Nozoe S, Hayashi M, Komiyama K
Department of Bioscience and Biotechnology, Aomori University, Japan.
Biol Pharm Bull. 2000 Feb;23(2):262-4. doi: 10.1248/bpb.23.262.
Four steroidal alkaloids, epipachysamines B (1) and E (2), pachystermine A (3) and pachysamine E (4), were isolated as cytotoxic principles from the MeOH extract of the stems of Pachysandra terminalis SIEB. et ZUCC. (Buxaceae). These alkaloids showed cytotoxic activity against P388 and P388/ADR leukemia cells in vitro. Three of the alkaloids (1-3) were previously isolated from this plant material, and this is the first report of their cytotoxic activity. Pachysamine E (4) is a new alkaloid.
从顶花板凳果（黄杨科）茎的甲醇提取物中分离出四种甾体生物碱，表板凳胺B（1）和E（2）、板凳明A（3）和板凳胺E（4），作为细胞毒性成分。这些生物碱在体外对P388和P388/ADR白血病细胞显示出细胞毒性活性。其中三种生物碱（1-3）先前已从该植物材料中分离得到，这是关于它们细胞毒性活性的首次报道。板凳胺E（4）是一种新生物碱。
