Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University , Tianjin 300070, People's Republic of China.
J Nat Prod. 2012 Jul 27;75(7):1305-11. doi: 10.1021/np300207c. Epub 2012 Jul 17.
The aim of the present study was to identify potentially useful natural compounds for the development of novel therapeutic agents to inhibit metastasis. A phytochemical investigation of Pachysandra terminalis resulted in the isolation of seven new pregnane alkaloids, terminamines A-G (1-7), and seven known alkaloids (8-14). The structures of 1-7 were elucidated by 1D- and 2D-NMR spectroscopic and mass spectrometric methods. Compounds 1-5 and 8-14 inhibited the migration of MB-MDA-231 breast cancer cells induced by the chemokine epithelial growth factor. In addition, compound 1 inhibited phosphorylation of integrin β(1), which plays an important role in MB-MDA-231 cell adhesion and metastasis.
本研究旨在鉴定具有潜在应用价值的天然化合物，以开发新型治疗药物来抑制转移。对铺地蜈蚣进行的植物化学研究导致了七种新的孕烷生物碱的分离，即terminamines A-G（1-7）和七种已知生物碱（8-14）。通过 1D-和 2D-NMR 光谱和质谱方法阐明了 1-7 的结构。化合物 1-5 和 8-14 抑制趋化因子上皮生长因子诱导的 MB-MDA-231 乳腺癌细胞的迁移。此外，化合物 1 抑制整合素 β(1)的磷酸化，整合素 β(1)在 MB-MDA-231 细胞黏附和转移中起着重要作用。
