Andreone Alessandro, de Hollander Daan
Burns Unit, Inkosi Albert Luthuli Central Hospital Durban, South Africa.
University of Kwa Zulu Natal, Nelson Mandela Medical School Durban, South Africa.
Int J Burns Trauma. 2020 Feb 15;10(1):15-20. eCollection 2020.
Different approaches can be used to repair extensive burn injury and chronic wounds, including full and split thickness skin grafts, temporising matrices and scaffolds, and composite cultured skin products. The use of non-cultured or autologous skin cells suspension in chronic burn is well established, but despite this no significant literature has been realized. The Rigenera micrografting technology is an innovative technique allowing to obtain a suspension of autologous micrografts that can be applied over the wounds in a combined methodology specifically developed and based on the both injections of the wound edges and spraying over the wound bed of this suspension. A black male patient with open wounds on the back already treated with a traditional split skin graft, present a 10% of wounds not healing. Then, the patient was treated with micrografts suspension obtained by mechanical disaggregation of small split skin biopsies using the Rigeneracons medical device. Micrografts were directly injected and sprayed in the wounds. The combination of injection and sprayed micrografts solution over the wounds achieved full closure over 10% over a period of 6 months. The follow up more than 2 years showed stable wounds with no breakdown in the epidermis. The final cosmetic and functional results obtained with micrografting on chronic burn wounds is a valid alternative when all the other options cannot provide wound closure.
可采用不同方法修复大面积烧伤和慢性伤口，包括全厚和中厚皮片移植、临时基质和支架以及复合培养皮肤产品。在慢性烧伤中使用非培养或自体皮肤细胞悬液已得到充分证实，但尽管如此，尚未出现大量相关文献。Rigenera微移植技术是一种创新技术，可获得自体微移植悬液，该悬液可通过专门开发的联合方法应用于伤口，该方法基于在伤口边缘注射以及将此悬液喷洒在伤口床面上。一名背部有开放性伤口的黑人男性患者，此前已接受传统中厚皮片移植治疗，仍有10%的伤口未愈合。然后，使用Rigeneracons医疗设备对小块中厚皮活检组织进行机械解离，获得微移植悬液对该患者进行治疗。微移植直接注射并喷洒在伤口上。在6个月的时间里，在伤口上注射和喷洒微移植溶液的组合实现了超过10%的伤口完全闭合。超过2年的随访显示伤口稳定，表皮无破损。当所有其他选择都无法实现伤口闭合时，在慢性烧伤伤口上进行微移植所获得的最终美容和功能效果是一种有效的替代方法。
