Zeevi M, Daniel V, Engelberg-Kulka H
Mol Gen Genet. 1979 Feb 26;170(2):149-53. doi: 10.1007/BF00337790.
Cell free extracts from a streptomycin-resistant E. coli mutant which is also temperature-sensitive for Q beta phage were studied for suppression of a nonsense mutation at various temperatures. The streptomycin-resistant ribosomes of the mutant were found to be temperature-sensitive in suppression of an amber mutation in f2 phage coat protein while retaining the ability to synthesize proteins at an elevated temperature (42 degrees C). The restriction of amber suppression at 42 degrees C is assumed to be related to an alteration in ribosomal protein S12 of the streptomycin-resistant mutant which also causes a change in its electrophoretic mobility.
对一株对链霉素耐药且对Qβ噬菌体温度敏感的大肠杆菌突变体的无细胞提取物进行了研究，以考察其在不同温度下对无义突变的抑制作用。发现该突变体的耐链霉素核糖体在抑制f2噬菌体外壳蛋白中的琥珀突变时对温度敏感，同时在高温（42摄氏度）下仍保留合成蛋白质的能力。推测42摄氏度下琥珀抑制作用的受限与耐链霉素突变体核糖体蛋白S12的改变有关，这种改变也导致其电泳迁移率发生变化。
