大肠杆菌16S rRNA 3'末端49个核苷酸片段二级结构的高分辨率质子磁共振研究。
High-resolution proton magnetic resonance study of the secondary structure of the 3'-terminal 49-nucleotide fragment of 16S rRNA from Escherichia coli.
作者信息
Baan R A, Hilbers C W, Van Charldorp R, Van Leerdam E, Van Knippenberg P H, Bosch L
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1028-31. doi: 10.1073/pnas.74.3.1028.
Abstract
The 3' terminus of 16S rRNA has been implicated in the recognition of mRNA's by the ribosome. A fragment containing the 3'-terminal 49 nucleotides cleaved from the rRNA by cloacin DF13 was isolated in a pure form. The secondary structure of this fragment has been studied by measuring the high-resolution proton magnetic resonance spectra. The resonances observed at low field can be assigned to hydrogen-bonded iminoprotons of base-pairs present in the fragment. From the data we conclude that the rRNA fragment, under the conditions used, exists as a hairpin consisting of eight intramolecular base-pairs, the 3'-terminal dodecanucleotide being unpaired. The implications of these findings with respect to the function of the ribosomal protein S1 are discussed.
摘要
16S核糖体RNA的3'末端与核糖体对信使RNA的识别有关。通过克洛辛DF13从核糖体RNA上切割下来的一个包含3'末端49个核苷酸的片段，以纯形式被分离出来。通过测量高分辨率质子磁共振谱对该片段的二级结构进行了研究。在低场观察到的共振可归因于该片段中存在的碱基对的氢键亚氨基质子。根据这些数据我们得出结论，在所使用的条件下，核糖体RNA片段以由八个分子内碱基对组成的发夹结构存在，3'末端的十二核苷酸未配对。讨论了这些发现对核糖体蛋白S1功能的影响。
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