Gupta Suphla, Pandotra Pankaj, Gupta Ajai P, Verma M K, Ahuja Ashok, Vishwakarma Ram A
1Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180 001 India.
2Quality Control and Assurance Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180 001 India.
Acta Physiol Plant. 2013;35(9):2699-2705. doi: 10.1007/s11738-013-1302-1. Epub 2013 May 21.
Direct rhizogenesis from leaf explants and establishment of an in vitro stolon culture system and subsequent plant regeneration for have been described. MS liquid medium supplemented with 0.01 mg l of NAA was most effective for stolon proliferation. Extensive proliferation of stolon and shoot regeneration was achieved on medium containing 3 % sucrose with 0.01 mg l NAA. Stolons with nodes showing growth was transferred under light for plantlet regeneration in the same medium. This paper is the first report in describing a complete regeneration procedure via in vitro stolon proliferation along with quantitative data for glycyrrhizin and genetic fidelity of plant regenerated in vitro there from. In vitro stolon proliferation described here would be an efficient way for regeneration of plants for functional genomics studies and better understanding of glycyrrhizin (GA) metabolism.
已经描述了从叶片外植体直接生根、建立体外匍匐茎培养系统以及随后的植株再生过程。添加了0.01 mg/L萘乙酸（NAA）的MS液体培养基对匍匐茎增殖最有效。在含有3%蔗糖和0.01 mg/L NAA的培养基上实现了匍匐茎的大量增殖和芽再生。带有生长节点的匍匐茎在光照条件下转移到相同培养基中进行植株再生。本文是第一篇描述通过体外匍匐茎增殖进行完整再生过程以及再生植株中甘草酸定量数据和遗传保真度的报告。这里描述的体外匍匐茎增殖将是一种有效的植物再生方法，用于功能基因组学研究以及更好地理解甘草酸（GA）代谢。
