Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan.
Plant Cell Rep. 1988 Dec;7(7):508-11. doi: 10.1007/BF00272743.
Callus and cell suspension cultures of Glycyrrhiza glabra failed to produce detectable amounts of glycyrrhizin, the major oleanane-type triterpene glycoside of the thickening root, or of its 11 -deoxoderivative. However, betulinic acid, a lupane-type triterpene, which was found in the root bark, and a small amount of β-amyrin, a possible precursor of oleanane-type triterpenes, were detected in cell suspension cultures in addition to lupeol, a fundamental form of lupane-type triterpenes. These findings suggest that the absence of glycyrrhizin in undifferentiated cultured cells may be partly due to interruption of the later reactions leading to the synthesis of glycyrrhizin from a triterpenoid intermediate.
甘草的愈伤组织和细胞悬浮培养物未能产生可检测量的甘草酸，甘草酸是增稠根中的主要齐墩果烷型三萜糖苷，或其 11-去氧衍生物。然而，白桦脂酸，一种羽扇豆烷型三萜，在根皮中发现，以及少量的β-香树脂醇，一种可能的齐墩果烷型三萜前体，除了羽扇豆烷型三萜的基本形式羽扇醇外，在细胞悬浮培养物中也被检测到。这些发现表明，在未分化的培养细胞中缺乏甘草酸可能部分是由于中断了导致从三萜类中间产物合成甘草酸的后期反应。
