Khan Saima, Pandotra Pankaj, Manzoor Malik Muzafar, Kushwaha Manoj, Sharma Rajni, Jain Shreyansh, Ahuja Ashok, Amancha Vishal, Bhushan Sashi, Guru Santosh Kumar, Gupta Ajai Prakash, Vishwakarma Ram, Gupta Suphla
1Plant Biology Division, Plant Biotechnology Department, Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir 180001 India.
2Quality Control and Quality Assurance Division, Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir 180001 India.
Plant Cell Tissue Organ Cult. 2016;124(3):507-516. doi: 10.1007/s11240-015-0910-4. Epub 2015 Dec 1.
Simultaneous qualitative and quantitative assessment of eight flavonoids and two terpenoids were performed in fourteen in vitro raised morphogenic cultures of . Our study revealed that the spectrum and production of ten compounds, under investigation, were higher in organized tissue than the undifferentiated mass, however, aerial portions of the in vitro raised plants (leaf and stem) were found to be devoid of therapeutically relevant triterpenoid, glycyrrhizin. A correlation was observed between cell maturation, morphological differentiation and glycyrrhizin accumulation. Mature stolons (4 months) were characterized by the maximum accumulation of glycyrrhizin (8.60 µg/mg) in in vitro plantlets. The cytotoxic effect of the extracts evaluated against a panel of human cancer cell lines (in vitro) indicated that the pancreatic cell line (MIA-PaCa-2) were sensitive to all the fourteen extracts investigated. To the best of our knowledge this is the first comprehensive report relating plant growth regulators to metabolite spectrum and cytotoxic assessment in in vitro raised cultures. Overall, our findings demonstrated that the metabolite spectrum of in vitro raised morphogenetic lines, under different stages of maturation, might offer a platform to understand the regulatory aspects of the concerned metabolite pathway and their consequent role in differentiation.
对十四种体外培养的形态发生培养物中的八种黄酮类化合物和两种萜类化合物进行了定性和定量同步评估。我们的研究表明，在所研究的十种化合物中，有组织的组织中的光谱和产量高于未分化的团块，然而，体外培养植物的地上部分（叶和茎）被发现不含具有治疗相关性的三萜类化合物甘草甜素。观察到细胞成熟、形态分化与甘草甜素积累之间存在相关性。成熟的匍匐茎（4个月）在体外幼苗中以甘草甜素的最大积累量（8.60μg/mg）为特征。对一组人类癌细胞系（体外）评估的提取物的细胞毒性作用表明，胰腺细胞系（MIA-PaCa-2）对所研究的所有十四种提取物敏感。据我们所知，这是第一份将植物生长调节剂与体外培养物中的代谢物谱和细胞毒性评估相关联的综合报告。总体而言，我们的研究结果表明，处于不同成熟阶段的体外培养形态发生系的代谢物谱可能为理解相关代谢物途径的调控方面及其在分化中的作用提供一个平台。
