Assaad Samir Naeim, Meheissen Marwa Ahmed, Elsayed Eman Tayae, Alnakhal Saher N, Salem Tarek M
Department of Internal Medicine, Endocrinology Unit, Faculty of Medicine, University of Alexandria, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Alexandria, Egypt.
J Clin Transl Endocrinol. 2020 Mar 12;20:100222. doi: 10.1016/j.jcte.2020.100222. eCollection 2020 Jun.
Hashimoto's thyroiditis (HT) is now considered one of the most prevalent autoimmune diseases. The aim of the present study was to determine the prevalence of different types of EBV antibodies in patients with HT in comparison to healthy controls, and to detect any correlation between EBV serological markers and different laboratory findings in HT patients.
SUBJECTS & METHODS: This case-control study was conducted on 120 subjects divided into two groups: Sixty patients with HT (patients group), and sixty age and sex matched healthy volunteers (control group). All the participants were subjected to: Thyroid ultrasound, laboratory assessment including: Serum thyroid -stimulating hormone (TSH), free tetraiodothyronine (FT4), free triiodothyronine (FT3), anti-thyroid peroxidase antibody (anti-TPO Ab) and anti-thyroglobulin antibody (anti-TG Ab). Four types of EBV antibodies (VCA IgM, VCA IgG, EA IgG, and EBNA-1IgG) were measured in serum using ELISA.
The mean serum levels of EBV VCA IgG and EA IgG were significantly higher in HT patients group in comparison to control group. In euthyroid HT patients, a significant positive correlation was observed between the age and EBV EA IgG. While in hypothyroid HT patients, a significant positive correlation between thyroid isthmus and EBNA-1IgG was observed. A significant negative correlation was found between the serum FT3 and EBNA-1IgG and a significant positive correlation was observed between serum TSH and EBV VCA IgG.
The high serum levels of EBV VCA IgG and EBV EA IgG in patients with HT suggest a possible association between EBV and HT.
桥本甲状腺炎（HT）现被认为是最常见的自身免疫性疾病之一。本研究的目的是确定HT患者中不同类型EBV抗体的患病率，并与健康对照进行比较，同时检测HT患者中EBV血清学标志物与不同实验室检查结果之间的相关性。
本病例对照研究共纳入120名受试者，分为两组：60例HT患者（患者组）和60名年龄及性别匹配的健康志愿者（对照组）。所有参与者均接受：甲状腺超声检查、实验室评估，包括：血清促甲状腺激素（TSH）、游离甲状腺素（FT4）、游离三碘甲状腺原氨酸（FT3）、抗甲状腺过氧化物酶抗体（抗-TPO Ab）和抗甲状腺球蛋白抗体（抗-TG Ab）。采用酶联免疫吸附测定法（ELISA）检测血清中四种类型的EBV抗体（VCA IgM、VCA IgG、EA IgG和EBNA-1IgG）。
与对照组相比，HT患者组血清中EBV VCA IgG和EA IgG的平均水平显著更高。在甲状腺功能正常的HT患者中，年龄与EBV EA IgG之间存在显著正相关。而在甲状腺功能减退的HT患者中，甲状腺峡部与EBNA-1IgG之间存在显著正相关。血清FT3与EBNA-1IgG之间存在显著负相关，血清TSH与EBV VCA IgG之间存在显著正相关。
HT患者血清中EBV VCA IgG和EBV EA IgG水平较高，提示EBV与HT之间可能存在关联。
