School of Chemistry and Chemical Engineering, School of Medicine, School of the Environment and Safety Engineering, Key Laboratory of Modern Agriculture Equipment and Technology, Jiangsu University, Zhenjiang, Jiangsu 212013, P. R. China.
Anal Chem. 2020 Apr 21;92(8):6072-6080. doi: 10.1021/acs.analchem.0c00492. Epub 2020 Apr 6.
Hypochlorite (ClO) and singlet oxygen (O) commonly coexist in living systems and exert important interplaying roles in many diseases. To dissect their complex inter-relationship, it is urgently required to construct a fluorescent probe that can discriminate ClO and O in living organisms. Herein, by taking the 3-(aliphaticthio)-propan-1-one group as the unique recognition unit for both ClO and O, we proposed the first fluorescent probe, , to simultaneously discriminate ClO and O with high sensitivity and selectivity. Probe itself showed fluorescence in blue channel. After treatment with ClO and O, respectively, pronounced fluorescence enhancements were observed in the green channel and red channel correspondingly. Moreover, upon development of the probe with aggregation-induced emission (AIE) characteristics, the probe could work well in a solution with high water volume fraction. Probe was also able to accumulate into mitochondria and was utilized as an effective tool to image exogenous and endogenous ClO and O in mitochondria. Significantly, as the first trial, probe was employed to simultaneously monitor the variation of ClO and O level in cecal tissues of rat in the cecal ligation and puncture (CLP)-induced polymicrobial sepsis model. The results demonstrated that the expressed ClO and O levels were tightly correlated with the severity of sepsis, inferring that the overproduction of ClO and O is an important factor in the pathogenesis of sepsis. The probe illustrated herein may provide a guide for further exploring the functions of ClO and O in various diseases.
次氯酸根（ClO）和单线态氧（O）通常共同存在于活体系中，并在许多疾病中发挥着重要的相互作用。为了剖析它们复杂的相互关系，迫切需要构建一种荧光探针，能够在活生物体中区分 ClO 和 O。在此，我们选择 3-（脂肪硫代）-1-丙酮基团作为 ClO 和 O 的唯一识别单元，设计并合成了第一个同时对 ClO 和 O 具有高灵敏度和选择性的荧光探针 。探针 本身在蓝色通道中显示荧光。分别用 ClO 和 O 处理后，在绿色通道和红色通道中分别观察到明显的荧光增强。此外，通过开发具有聚集诱导发射（AIE）特性的探针，该探针可以在高水体积分数的溶液中很好地工作。探针 还能够积累在线粒体中，并被用作有效工具来成像线粒体中外源和内源性 ClO 和 O。值得注意的是，作为首次尝试，探针 被用于同时监测盲肠结扎和穿刺（CLP）诱导的多微生物脓毒症模型中大鼠盲肠组织中 ClO 和 O 水平的变化。结果表明，表达的 ClO 和 O 水平与脓毒症的严重程度密切相关，这表明 ClO 和 O 的过度产生是脓毒症发病机制中的一个重要因素。本文所述的探针可能为进一步探索 ClO 和 O 在各种疾病中的功能提供指导。
