Liu Xu, Liu Xin, Shen Youming, Gu Biao
Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.
Key Laboratory of Functional Organometallic Materials of College of Hunan Province, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, P. R. China.
ACS Omega. 2020 Aug 19;5(34):21684-21688. doi: 10.1021/acsomega.0c02589. eCollection 2020 Sep 1.
Developing an effective method for monitoring fluoride ion in biological samples is meaningful because fluoride ion plays a vital role in biological processes. In this contribution, a simple water-soluble ESIPT fluorescent probe 2-((4-((-butyldiphenylsilyl)oxy)-1,3-dioxoisoindolin-2-yl)methyl)-1-ethylpyridin-1-ium iodide () was constructed for monitoring fluoride ion. The probe containing pyridinium salt group exhibited preeminent water solubility. The probe introducing a trimethyldiphenylsilyl ether recognition group displayed excellent selectivity for fluoride ion over other biologically relevant species. Additionally, the probe exhibited a fast response for a fluoride ion, suggesting that it could provide real-time fluoride ion detection. Importantly, the probe could detect fluoride ion with a linear range of 0-70.0 × 10 M and a low detection limit of 1.16 × 10 M. Furthermore, probe could detect fluoride ion with a large Stokes shift (98 nm), which was attributed to ESIPT fluorescence sensing process. At last, probe was successfully employed to monitor fluoride ion in living cells.
开发一种有效的生物样品中氟离子监测方法具有重要意义，因为氟离子在生物过程中起着至关重要的作用。在本研究中，构建了一种简单的水溶性ESIPT荧光探针2-((4-((-丁基二苯基甲硅烷基)氧基)-1,3-二氧代异吲哚啉-2-基)甲基)-1-乙基吡啶碘化物()用于监测氟离子。含有吡啶盐基团的探针表现出卓越的水溶性。引入三甲基二苯基甲硅烷基醚识别基团的探针相对于其他生物相关物种对氟离子表现出优异的选择性。此外，该探针对氟离子表现出快速响应，表明它可以提供实时氟离子检测。重要的是，该探针能够检测氟离子，线性范围为0-70.0×10 M，检测限低至1.16×10 M。此外，探针能够以较大的斯托克斯位移(98 nm)检测氟离子，这归因于ESIPT荧光传感过程。最后，探针成功用于监测活细胞中的氟离子。
