Filatov L V, Mamayeva S E, Tomilin N V
Institute of Cytology, Academy of Sciences of the USSR, Leningrad.
Mol Biol Rep. 1988;13(2):79-84. doi: 10.1007/BF00539054.
The distribution of Alu-family DNA repeats (AFRs) in chromosomes of phytohaemagglutinin-stimulated peripheral blood lymphocytes of four normal donors and non-stimulated bone marrow cells of four patients with acute leukemia (ALL and ANLL) was studied by in situ hybridization using DNA of recombinant phage lambda containing multiple inserts of AFR as a probe. Over some chromosome bands (14cen, 16p13, 16cen) from normal donors and from leukemic patients clusters of silver grains were detected. Over other three bands (3q26, 8p11-p12 and 14q24) the clusters were found only in chromosomes from the four acute leukemia patients, and were absent from chromosomes of healthy donors. The results suggest non-random long-range distribution of AFRs in human chromosomes, and somatic variations in the distribution of the repeats.
采用含多个Alu家族DNA重复序列（AFR）插入片段的重组λ噬菌体DNA作为探针，通过原位杂交技术研究了4名正常供体经植物血凝素刺激的外周血淋巴细胞以及4名急性白血病（急性淋巴细胞白血病和急性非淋巴细胞白血病）患者未受刺激的骨髓细胞染色体中AFR的分布情况。在正常供体和白血病患者的某些染色体带（14cen、16p13、16cen）上检测到了银粒簇。在另外三条染色体带（3q26、8p11 - p12和14q24）上，仅在4名急性白血病患者的染色体中发现了银粒簇，而健康供体的染色体中未出现。结果表明AFR在人类染色体中呈非随机的远距离分布，且重复序列的分布存在体细胞变异。
