Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology and Optometry/School of Biomedical Engineering, State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
J Mater Chem B. 2020 May 7;8(17):3842-3851. doi: 10.1039/c9tb02814e. Epub 2020 Mar 27.
Developing a biomaterial that can promote osteoblastic differentiation, thereby reducing the needs of exogenous osteogenic factors for large bone repair, has been a significant and long-term technical hurdle. In this study, we developed an innovative nanoclay (nanosilicate, NS)-functionalized 3D gelatin nanofibrous scaffold (GF/NS) through a thermally induced phase separation method together with the particle leaching technique (TIPS&P). In addition to the significantly higher mechanical strength, the composite scaffolds (GF/NS) demonstrated a significantly stronger ability to promote the osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro compared to the GF scaffold. Our data further revealed that this intriguing pro-osteoblastic functionality was largely because of the unique features of NS, particularly, the strong binding ability to pro-osteoblastic factors (e.g., BMP2) as well as the intrinsic osteoinductivity of its bioactive degradation products. Most importantly, our in vivo studies indicated that GF/NS scaffolds significantly improved low-dose BMP2-induced ectopic bone regeneration in mice.
开发一种能够促进成骨细胞分化的生物材料，从而减少大骨修复对外部成骨因子的需求，一直是一个重大且长期的技术难题。在这项研究中，我们通过热致相分离法和颗粒沥滤技术（TIPS&P）开发了一种创新的纳米粘土（纳米硅酸钠，NS）功能化 3D 明胶纳米纤维支架（GF/NS）。除了显著提高机械强度外，与 GF 支架相比，复合支架（GF/NS）在体外显著增强了人骨髓间充质干细胞（hMSC）的成骨分化能力。我们的数据进一步表明，这种有趣的促成骨功能主要归因于 NS 的独特特性，特别是其与促成骨因子（例如 BMP2）的强结合能力以及其生物活性降解产物的固有成骨诱导性。最重要的是，我们的体内研究表明，GF/NS 支架显著改善了低剂量 BMP2 诱导的小鼠异位骨再生。
