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接种剂对青藏高原披碱草(Phalaris arundinacea L.)青贮饲料发酵特性及体外消化率的影响

Effects of inoculants on the fermentation characteristics and in vitro digestibility of reed canary grass (Phalaris arundinacea L.) silage on the Qinghai-Tibetan Plateau.

作者信息

Chen Liangyin, Li Ping, Gou Wenlong, You Minghong, Cheng Qiming, Bai Shiqie, Cai Yimin

机构信息

Sichuan Academy of Grassland Sciences, Chengdu, China.

Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, Japan.

出版信息

Anim Sci J. 2020 Jan-Dec;91(1):e13364. doi: 10.1111/asj.13364.

Abstract

To effectively use local available grass resources to cover the winter feed shortage on the Qinghai-Tibetan Plateau, direct-cut and wilted reed canary grass (RCG) silages were prepared by using a rolled-bale system, and their ensiling characteristics and in vitro digestibility were studied. Silages were treated without (control) or with inoculants including LP (Lactobacillus plantarum), LPLB (L. plantarum, L. buchneri), and LPLBc (L. plantarum, L. buchneri, and cellulase), and were stored at ambient temperature (5.7-14.6°C) for 90 days. Compared with control, the inoculated silages increased (p < .05) lactic acid and acetic acid contents, and reduced (p < .05) final pH value and ammonia-N ratio of total N. The highest WSC content (41.2 g/kg DM) occurred for LPLB-inoculated silage, whereas LPLBc-treated silage displayed the lowest contents of NDF (522.9 g/kg DM) and ADF (275.5 g/kg DM). In addition, LPLBc-inoculated silage had the highest in vitro gas production (51.0 ml/g DM), in vitro DM digestibility (619.3 g/kg DM), and metabolic energy (9.6 kJ/kg DM). These results confirmed that treatments with inoculants at ensiling could improve silage fermentation and in vitro digestibility of RCG, and this could be a potential winter feed for animals on the Qinghai-Tibetan Plateau.

摘要

为有效利用当地现有的牧草资源来弥补青藏高原冬季饲料短缺的问题,采用裹包青贮系统制备了直接刈割并萎蔫的披碱草青贮料,并对其青贮特性和体外消化率进行了研究。青贮料分为不添加任何处理(对照)以及添加包括LP(植物乳杆菌)、LPLB(植物乳杆菌、布氏乳杆菌)和LPLBc(植物乳杆菌、布氏乳杆菌和纤维素酶)的接种剂处理组,在环境温度(5.7 - 14.6°C)下储存90天。与对照组相比,接种青贮料提高了(p < 0.05)乳酸和乙酸含量,并降低了(p < 0.05)最终pH值和氨态氮占总氮的比例。接种LPLB的青贮料水溶性碳水化合物(WSC)含量最高(41.2 g/kg干物质),而经LPLBc处理的青贮料中性洗涤纤维(NDF)(522.9 g/kg干物质)和酸性洗涤纤维(ADF)(275.5 g/kg干物质)含量最低。此外,接种LPLBc的青贮料体外产气量最高(51.0 ml/g干物质)、体外干物质消化率最高(619.3 g/kg干物质)、代谢能最高(9.6 kJ/kg干物质)。这些结果证实,青贮时接种处理可改善披碱草青贮料的发酵和体外消化率,这可能是青藏高原动物潜在的冬季饲料。

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