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添加剂对青藏高原不同成熟阶段多年生燕麦青贮发酵特性及体外消化率的影响

Effects of Additives on Silage Fermentation Characteristic and In Vitro Digestibility of Perennial Oat at Different Maturity Stages on the Qinghai Tibetan.

作者信息

Li Ping, Tang Xiaolong, Liao Chaosheng, Li Maoya, Chen Liangyin, Lu Guangrou, Huang Xiaokang, Chen Chao, Gou Wenlong

机构信息

College of Animal Science, Guizhou University, Guiyang 550025, China.

Sichuan Academy of Grassland Sciences, Chengdu 611731, China.

出版信息

Microorganisms. 2021 Nov 22;9(11):2403. doi: 10.3390/microorganisms9112403.

Abstract

To effectively use local grass resources to cover the winter feed shortage on the Qinghai-Tibetan Plateau, the silage fermentation and in vitro digestibility of perennial oat ( Henr.) were investigated. Perennial oat was harvested at the heading/flowering stage, wilted under sunny conditions, chopped, vacuumed in small bag silos, and stored at ambient temperatures (5-15 °C) for 60 days. The silages were treated without (CK) or with local lactic acid bacteria (LAB) inoculant (IN1), commercial LAB inoculant (IN2), and sodium benzoate (BL). Control silages of perennial oat at early heading stage showed higher ( < 0.05) lactate and acetate contents and lower ( < 0.05) final pH, butyrate, and ammonia-N contents than those at the flowering stage. High levels of dry matter recovery (DMR) and crude protein (CP) were observed in IN1- and BL-treated silages, with high in vitro gas production and dry matter digestibility. Compared to CK, additives increased ( < 0.05) aerobic stability by inhibiting yeasts, aerobic bacteria, and coliform bacteria during ensiling. In particular, the local LAB inoculant increased ( < 0.05) concentrations of lactate, acetate and propionate, and decreased concentrations of butyrate and ammonia-N in silages. This study confirmed that local LAB inoculant could improve the silage quality of perennial oat, and this could be a potential winter feed for animals such as yaks on the Qinghai Tibetan Plateau.

摘要

为有效利用当地牧草资源弥补青藏高原冬季饲料短缺,对多年生燕麦(Henr.)的青贮发酵及体外消化率进行了研究。多年生燕麦在抽穗/开花期收获,在晴天条件下萎蔫,切碎后装入小型袋装青贮窖进行真空处理,并在环境温度(5 - 15℃)下储存60天。青贮饲料分别进行不添加处理(CK)、添加当地乳酸菌(LAB)接种剂(IN1)、商业LAB接种剂(IN2)以及苯甲酸钠(BL)的处理。多年生燕麦早期抽穗期的对照青贮饲料比开花期的青贮饲料具有更高(<0.05)的乳酸和乙酸含量以及更低(<0.05)的最终pH值、丁酸和氨态氮含量。在IN1和BL处理的青贮饲料中观察到高水平的干物质回收率(DMR)和粗蛋白(CP),体外产气和干物质消化率也较高。与CK相比,添加剂通过抑制青贮过程中的酵母菌、好氧细菌和大肠菌增加了(<0.05)有氧稳定性。特别是,当地LAB接种剂增加了(<0.05)青贮饲料中乳酸、乙酸和丙酸的浓度,并降低了丁酸和氨态氮的浓度。本研究证实,当地LAB接种剂可提高多年生燕麦的青贮质量,这可能是青藏高原牦牛等动物潜在的冬季饲料。

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