College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Genomics. 2020 Jul;112(4):2804-2812. doi: 10.1016/j.ygeno.2020.03.018. Epub 2020 Mar 24.
Myogenic factor 5 plays actively roles in the regulation of myogenesis. The aims of this study are to identify the evolution information of MYF5 protein among 10 domestic and mammalian animals, to uncover the expression patterns of MYF5 gene in calves and adults of Qinchuan cattle, and to expose the genetic variants of the MYF5 gene and explore its effect on cattle growth traits and beef quality traits in Qinchuan cattle. The bioinformatics results showed that the MYF5 proteins highly conserved in different mammalian or domestic animals apart from chicken. The expression level of MYF5 gene in the heart, muscle, lung, large intestine and liver was greater than that of other tissues. PCR amplicons sequencing identified four novel SNPs at g.5738A>G, g.5785C>T and g.5816A>G in the 3rd exon region and g.6535A>G in the 3' UTR. Genotypic frequencies of g.5785C>T was harshly deviated from the HWE (P < .05). Genetic diversity was low or intermediate for the four SNPs and those SNPs were in the weak linkage disequilibrium. Association analysis results indicated g.5785C>T, g.5816A>G and g.6535A>G significant effect on growth performance and beef quality traits of Qinchuan cattle. H1H3 diplotype had greater body size and better beef quality. All the results implicate that the MYF5 gene might be applied as a promising candidate gene in Qinchuan cattle breeding.
肌生成因子 5 在调节肌发生中发挥着积极的作用。本研究的目的是鉴定 10 种家畜和哺乳动物中 MYF5 蛋白的进化信息，揭示 MYF5 基因在秦川牛犊牛和成牛中的表达模式，并揭示 MYF5 基因的遗传变异及其对秦川牛生长和肉质性状的影响。生物信息学结果表明，除鸡以外，不同哺乳动物或家畜的 MYF5 蛋白高度保守。MYF5 基因在心、肌肉、肺、大肠和肝脏中的表达水平高于其他组织。PCR 扩增子测序在第 3 外显子区域的 g.5738A>G、g.5785C>T 和 g.5816A>G 以及 3'UTR 中的 g.6535A>G 处鉴定出四个新的 SNP。g.5785C>T 的基因型频率严重偏离 HWE（P<.05）。四个 SNP 的遗传多样性低或中等，且这些 SNP 处于弱连锁不平衡状态。关联分析结果表明，g.5785C>T、g.5816A>G 和 g.6535A>G 对秦川牛生长性能和肉质性状有显著影响。H1H3 二倍型体型较大，肉质较好。所有结果表明，MYF5 基因可能作为秦川牛育种的一个有前途的候选基因。
