Modern Research Center for Traditional Chinese Medicine, Institute of Molecular Science, Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, China.
School of Traditional Chinese Materia Medica, Shanxi University of Chinese Medicine, No. 121, Daxue Street, Taiyuan 030619, Shanxi, China.
Food Chem. 2020 Aug 1;320:126651. doi: 10.1016/j.foodchem.2020.126651. Epub 2020 Mar 19.
Ziziphi Spinosae Folium, the leaf of Ziziphus jujuba Mill. Var. spinosa (Bunge) Hu ex H. F. Chou (LZJS), is currently used as a healthy tea in China. This study evaluated the chemical components and antioxidant activities of LZJS flavonoid (LZJSF) and fermented LZJSF (FLZJSF) using human intestinal bacteria (HIB) through dynamic fermentation. Eighteen flavonoids were simultaneously identified in LZJSF using UHPLC-Q-Orbitrap-MS method, nine of which were targeted for a HIB metabolism study. Seven small phenolic acids were identified in FLZJSF. Not only at chemical level but also at PC12 cell level, FLZJSF samples fermented for 4 and 6 h showed significant positive correlation between their activities and flavonoid aglycones, which were transformed from LZJSF. However, FLZJSF samples (8 h and longer time) mainly contained phenolic acids and indicated weak activities. Thus, LZJSF was found to result in increased antioxidant activity and could be commercially utilized as a novel functional food.
酸枣叶，是鼠李科枣属植物酸枣的叶（君迁子 var. spinosa (Bunge) Hu ex H. F. Chou），目前在中国被用作健康茶。本研究通过动态发酵用人肠道细菌（HIB）评估了LZJS 黄酮（LZJSF）和发酵的 LZJSF（FLZJSF）的化学成分和抗氧化活性。使用 UHPLC-Q-Orbitrap-MS 方法同时鉴定了 LZJSF 中的 18 种黄酮类化合物，其中 9 种被用于 HIB 代谢研究。在 FLZJSF 中鉴定出了 7 种小酚酸。不仅在化学水平上，而且在 PC12 细胞水平上，发酵 4 和 6 小时的 FLZJSF 样品与其活性和从 LZJSF 转化而来的黄酮苷元之间表现出显著的正相关。然而，FLZJSF 样品（8 小时及更长时间）主要含有酚酸，活性较弱。因此，发现 LZJSF 可提高抗氧化活性，可作为一种新型功能性食品进行商业利用。
