Damiano Silvia, Forino Martino, De Arpan, Vitali Luca A, Lupidi Giulio, Taglialatela-Scafati Orazio
School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, Italy.
Department of Pharmacy, University of Naples Federico II, Via Montesano 49, 80131 Naples, Italy.
Food Chem. 2017 Sep 1;230:24-29. doi: 10.1016/j.foodchem.2017.02.141. Epub 2017 Mar 3.
Ziziphus jujuba Mill., jujube, leaf infusions are popular bedtime beverages as they improve sleep by soothing the nerves. With the aim of providing further insights into the polyphenol content of jujube leaf infusion and their antioxidant activities, a 2,2-diphenyl-1-picrylhydrazyl (DPPH)-guided purification of the aqueous extract of Z. jujuba Mill. leaves was conducted. Three major antioxidant polyphenols (i.e. quercitrin, catechin and gallocatechin) of the leaves were identified. In addition, a significant antibiofilm bioactivity against Streptococcus mutans, a causative agent of human dental caries, was detected for the aqueous leaf extract and its bio-guided separation resulted in the identification of the lupane triterpenoid alphitolic acid as the main antibiofilm metabolite. The results herein presented further promote the consumption of jujube leaf infusion as a healthy antioxidant bedtime beverage, and associate it to an unreported anti-caries activity.
酸枣（Ziziphus jujuba Mill.）叶泡的茶是受欢迎的睡前饮品，因为它们能舒缓神经，改善睡眠。为了进一步深入了解酸枣叶茶中的多酚含量及其抗氧化活性，对酸枣叶的水提取物进行了2,2-二苯基-1-苦基肼（DPPH）引导的纯化。鉴定出了酸枣叶中的三种主要抗氧化多酚（即槲皮苷、儿茶素和表没食子儿茶素）。此外，检测到酸枣叶水提取物对人类龋齿的致病菌变形链球菌具有显著的抗生物膜生物活性，并且通过其生物导向分离鉴定出羽扇豆烷三萜α-香树脂酸是主要的抗生物膜代谢产物。本文的研究结果进一步推动了酸枣叶茶作为一种健康的抗氧化睡前饮品的消费，并将其与一种未报道的抗龋齿活性联系起来。
