Zhang Zeyu, Wang Zhiming, Huang Yun
Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Medicine (Baltimore). 2020 Mar;99(13):e19606. doi: 10.1097/MD.0000000000019606.
Intrahepatic cholangiocarcinoma (ICC) is an aggressive biliary epithelial tumor with poor prognosis. There are increasing evidences that long non-coding RNAs (lncRNAs) are dysregulated in multifarious tumors, revealing potential significant role of lncRNAs in tumorigenesis.We used the ICC dataset retrieved from The Cancer Genome Atlas and the Gene Expression Omnibus database to obtain the lncRNAs expression profiles and identify potential prognostic lncRNAs for predicting the prognosis in ICC. Univariate and multivariate Cox regression analyses were performed to construct a prognostic index (PI). Furthermore, coexpression analysis and functional assessment were performed to initially investigate the function of these prognostic lncRNAs.A total of 255 differentially expressed lncRNAs (DElncRNAs) were identified among two RNA sequencing dataset of a total 63 ICC patients with 98 samples using R platform. Thirteen of 255 DElncRNAs were identified as prognostic lncRNAs and used for a PI. Patients with high PI were associated with poor prognostic (P = .0064), and the Cox regression showed consistent result (P = .042). The time-dependent receiver operating characteristic analysis showed the PI performed well in ICC survival prediction with an area under curve of 0.921, 0.801, and 0.717 for 1-, 3-, and 5-year survival, respectively.In conclusion, we included 13 identified prognostic DElncRNAs and constructed a prognostic signature/PI. ICC patient with higher PI was associated with poorer prognosis. However, the clinical role as well as biological functions of constructed PI and these prognostic DElncRNAs need to be verified in future study.
肝内胆管癌（ICC）是一种侵袭性胆管上皮肿瘤，预后较差。越来越多的证据表明，长链非编码RNA（lncRNAs）在多种肿瘤中表达失调，揭示了lncRNAs在肿瘤发生中的潜在重要作用。我们使用从癌症基因组图谱和基因表达综合数据库中检索到的ICC数据集来获取lncRNAs表达谱，并识别潜在的预后lncRNAs，以预测ICC的预后。进行单变量和多变量Cox回归分析以构建预后指数（PI）。此外，进行共表达分析和功能评估以初步研究这些预后lncRNAs的功能。使用R平台在总共63例ICC患者的98个样本的两个RNA测序数据集中共鉴定出255个差异表达的lncRNAs（DElncRNAs）。255个DElncRNAs中的13个被鉴定为预后lncRNAs并用于构建PI。PI高的患者预后较差（P = 0.0064），Cox回归显示出一致的结果（P = 0.042）。时间依赖性受试者工作特征分析表明，PI在ICC生存预测中表现良好，1年、3年和5年生存率的曲线下面积分别为0.921、0.801和0.717。总之，我们纳入了13个鉴定出的预后DElncRNAs并构建了一个预后特征/PI。PI较高的ICC患者预后较差。然而，构建的PI以及这些预后DElncRNAs的临床作用和生物学功能需要在未来的研究中得到验证。
