Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing, 400715, People's Republic of China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, People's Republic of China.
Mikrochim Acta. 2021 Aug 29;188(9):312. doi: 10.1007/s00604-021-04977-w.
Enzymes have demonstrated great potential in the development of advanced electroanalysis devices due to their unique recognition and catalytic properties. However, unsatisfactory stability and limited electron communication of traditional enzyme sensors seriously hinder their large-scale application. In this work, a simple and effective method is proposed to improve the stability of enzyme sensors by using sodium hyaluronate (SH) as a protective film, MXene-TiC/Glucose oxidase (GOD) as the reaction layer, and chitosan (CS) /reduced graphene oxide (rGO) as the adhesion layer. Results demonstrate that the repeatability of the designed sensor increased by 73.3% after improving the adhesion between the reaction layer and the current collector and that its response ability was greatly enhanced. Moreover, the long-term stability of the electrode surface with SH protective film proved to be superior than that without protective film, which suggests that this design can effectively improve the overall performance of the enzyme biosensor. This work proposed a multi-tier synergistic approach for improving the reliability of enzyme sensors. Graphical abstract Our proposed protective and adhesion layer can greatly improve the stability of enzyme sensor and realize the rapid detection of glucose in serum sample.
由于其独特的识别和催化特性，酶在先进的电分析器件的发展中表现出巨大的潜力。然而，传统酶传感器稳定性差和电子通信有限严重阻碍了其大规模应用。在这项工作中，提出了一种简单有效的方法，通过使用透明质酸钠 (SH) 作为保护膜、MXene-TiC/葡萄糖氧化酶 (GOD) 作为反应层和壳聚糖 (CS)/还原氧化石墨烯 (rGO) 作为粘附层来提高酶传感器的稳定性。结果表明，通过改善反应层与集流器之间的附着力，设计的传感器的重复性提高了 73.3%，其响应能力得到了极大增强。此外，具有 SH 保护膜的电极表面的长期稳定性优于没有保护膜的电极表面，这表明这种设计可以有效地提高酶生物传感器的整体性能。这项工作提出了一种多层协同方法来提高酶传感器的可靠性。
