Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
J Microbiol. 2019 Feb;57(2):122-126. doi: 10.1007/s12275-019-8562-4. Epub 2019 Jan 31.
A yellow-colored bacterium with gliding motility, strain KIS68-18, was isolated from a soil sample at Bijin Island in Tongyeong city, Republic of Korea. The cells were strictly aerobic, Gram-staining-negative, non-spore-forming, and rod-shaped. The strain grew at the range of 10-35°C (optimum, 25-30°C), pH 5.5-8.0 (optimum, 6.0-7.5), and 0-0.5% (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KIS68-18 was closely related to Chryseolinea serpens DSM 24574 (98.9%) and had low sequence similarities (below 92.6%) with other members of the family 'Cytophagaceae' in the phylum Bacteroidetes. The major respiratory quinone system was MK-7 and the predominant cellular fatty acids were Cω5c (38.8%), iso-C (18.5%), and summed feature 3 (Cω7c and/or Cω6c, 10.6%). The polar lipids consisted of phosphatidylethanolamine, one unidentified phospholipid, three unidentified aminophospholipids, two unidentified aminolipids, and five unidentified lipids. The DNA G + C content was 50.9%. Based on the phylogenetic, physiological, and chemotaxonomic data, stain KIS68-18 represents a novel species of the genus Chryseolinea, for which the name Chryseolinea soli sp. nov. is proposed. The type strain of Chryseolinea soli is KIS68-18 (= KACC 17327 = NBRC 113100).
一株具有滑行运动能力的黄色细菌，菌株 KIS68-18，从韩国统营市美人岛的土壤样本中分离得到。细胞严格需氧，革兰氏染色阴性，不形成孢子，呈杆状。该菌株在 10-35°C（最佳温度 25-30°C）、pH5.5-8.0（最佳 pH6.0-7.5）和 0-0.5%（w/v）NaCl 的范围内生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KIS68-18与 Chryseolinea serpens DSM 24574（98.9%）密切相关，与厚壁菌门“噬细胞科”的其他成员的序列相似性较低（低于 92.6%）。主要呼吸醌系统为 MK-7，主要细胞脂肪酸为 Cω5c（38.8%）、iso-C（18.5%）和总和特征 3（Cω7c 和/或 Cω6c，10.6%）。极性脂类由磷脂酰乙醇胺、一种未鉴定的磷脂、三种未鉴定的氨基磷脂、两种未鉴定的氨基脂类和五种未鉴定的脂类组成。DNA G+C 含量为 50.9%。基于系统发育、生理和化学分类学数据，菌株 KIS68-18代表 Chryseolinea 属的一个新种，建议将其命名为 Chryseolinea soli sp. nov.。Chryseolinea soli 的模式菌株为 KIS68-18（=KACC 17327=NBRC 113100）。
