Phenyllactic Acid Produced by Reduces and Growth and T-2 Toxin Concentration.

and are present in barley crops. Their toxic metabolites, mainly T-2 toxin, affect the quality and safety of raw material and final products such as beer. Therefore, it is crucial to reduce . proliferation and T-2 toxin contamination during the brewing process. The addition of has been previously demonstrated to reduce the proliferation of . and the production of toxic metabolites, but the mechanism of action is still not known. Thus, this study focuses on the elucidation of the interaction mechanism between and . in order to improve this bioprocess. First, over a period of 168 h, the co-culture kinetics showed an almost 90% reduction in T-2 toxin concentration, starting at 24 h. Second, sequential cultures lead to a reduction in growth and T-2 toxin concentration. Simultaneously, it was demonstrated that produces phenyllactic acid (PLA) at the early stages of growth, which could potentially be responsible for the reduction in growth and T-2 toxin concentration. To prove the PLA effect, and were cultivated in PLA supplemented medium. The expected results were achieved with 0.3 g/L of PLA. These promising results contribute to a better understanding of the bioprocess, allowing its optimization at an up-scaled industrial level.