Sun Han, Zhang Wenping, Wu Yongzhen, Gao Lifeng, Cui Fa, Zhao Chunhua, Guo Zhiai, Jia Jizeng
College of Agriculture, Ludong University, Yantai, China.
Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, Ludong University, Yantai, China.
Front Plant Sci. 2020 Mar 12;11:285. doi: 10.3389/fpls.2020.00285. eCollection 2020.
Timing of flowering is crucial for the transformation from vegetative to reproductive growth in the important food crop, wheat ( L.). The circadian clock is a central part of photoperiod regulation, with Pseudo-Response Regulators (PRRs) representing key components of circadian networks. However, little is known about the effects of PRR family members on yield-related traits in crop plants. In this study, we identified polymorphisms and haplotypes of , demonstrating that natural variations in are associated with significant differences in yield-related traits including heading date, plant height and thousand grain weight. showed an earlier heading date, advanced by 0.9 to 1.7%. and displayed reduced plant height and increased thousand grain weight of up to 13.3 to 26.4% and 6.3 to 17.3%, respectively. Subcellular localization and transcriptional activity analysis showed that TaPRR1 is a nuclear localization protein with transcriptional activity controlled by an IR domain. The expression profiles of genes over a 48-h period were characterized by circadian rhythms, which had two peaks under both short- and long- day conditions. In addition, geographical distribution analysis indicated higher distribution frequencies of , , and in different agro-ecological production zones. Furthermore, analysis of molecular variance of the distribution frequency of haplotypes suggested significant differences in haplotype distribution frequency between landraces and modern cultivars. Our study provides a basis for in-depth understanding of function on yield-related traits in wheat, as well as establishing theoretical guidance for wheat molecular marker-assisted breeding.
开花时间对于重要粮食作物小麦从营养生长向生殖生长的转变至关重要。生物钟是光周期调控的核心部分，伪响应调节因子（PRRs）是生物钟网络的关键组成部分。然而，关于PRR家族成员对作物产量相关性状的影响知之甚少。在本研究中，我们鉴定了（此处原文缺失具体基因名称）的多态性和单倍型，表明（此处原文缺失具体基因名称）的自然变异与包括抽穗期、株高和千粒重在内的产量相关性状的显著差异有关。（此处原文缺失具体基因名称）表现出更早的抽穗期，提前了0.9%至1.7%。（此处原文缺失具体基因名称）和（此处原文缺失具体基因名称）的株高降低，千粒重分别增加了13.3%至26.4%和6.3%至17.3%。亚细胞定位和转录活性分析表明，TaPRR1是一种具有由IR结构域控制的转录活性的核定位蛋白。（此处原文缺失具体基因名称）基因在48小时内的表达谱具有昼夜节律特征，在短日照和长日照条件下均有两个峰值。此外，地理分布分析表明，（此处原文缺失具体基因名称）、（此处原文缺失具体基因名称）和（此处原文缺失具体基因名称）在不同农业生态生产区的分布频率较高。此外，对（此处原文缺失具体基因名称）单倍型分布频率的分子方差分析表明，地方品种和现代品种之间的单倍型分布频率存在显著差异。我们的研究为深入了解（此处原文缺失具体基因名称）对小麦产量相关性状的功能提供了基础，也为小麦分子标记辅助育种建立了理论指导。
