Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Medical Scientist Training Program, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Regen Med. 2020 Feb;15(2):1295-1312. doi: 10.2217/rme-2019-0084. Epub 2020 Mar 31.
To evaluate dermal regeneration scaffolds custom-fabricated from fibril-forming oligomeric collagen where the total content and spatial gradient of collagen fibrils was specified. Microstructural and mechanical features were verified by electron microscopy and tensile testing. The ability of dermal scaffolds to induce regeneration of rat full-thickness skin wounds was determined and compared with no fill control, autograft skin and a commercial collagen dressing. Increasing fibril content of oligomer scaffolds inhibited wound contraction and decreased myofibroblast marker expression. Cellular and vascular infiltration of scaffolds over the 14-day period varied with the graded density and orientation of fibrils. Fibril content, spatial gradient and orientation are important collagen scaffold design considerations for promoting vascularization and dermal regeneration while reducing wound contraction.
评估由纤维形成的低聚胶原定制制造的真皮再生支架，其中胶原纤维的总含量和空间梯度被指定。通过电子显微镜和拉伸试验验证了微观结构和机械性能。通过确定真皮支架诱导大鼠全层皮肤伤口再生的能力，并将其与无填充对照、自体移植物皮肤和商业胶原敷料进行比较。增加低聚支架的纤维含量可抑制伤口收缩并减少肌成纤维细胞标志物的表达。在 14 天的时间内，支架的细胞和血管浸润随纤维的分级密度和方向而变化。纤维含量、空间梯度和方向是促进血管生成和皮肤再生同时减少伤口收缩的重要胶原支架设计考虑因素。
