Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China; National Clinical Research Center for Oral Diseases, Beijing, China; National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China; Beijing Key Laboratory of Digital Stomatology, Beijing, China.
National Clinical Research Center for Oral Diseases, Beijing, China; National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China; Beijing Key Laboratory of Digital Stomatology, Beijing, China; Center of Digital Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110530. doi: 10.1016/j.msec.2019.110530. Epub 2019 Dec 6.
Peripheral nerve injuries often cause different degrees of sensory and motor function loss. Currently, the repair effect of the "gold standard", autologous nerve transplantation, is unsatisfactory. Tissue engineering has the potential to tissue manipulation, regeneration, and growth, but achieving personalization and precision remains a challenge. In this study, we used 3D bioprinting to construct a nerve scaffold composed of gelatin/alginate hydrogel containing rat Schwann cells. On day 1 after printing, the Schwann cell survival rate was 91.87 ± 0.55%. Cells could be cultured in the hydrogel for 7 days, and were well attached to the surface of the scaffold. On days 4 and 7, the 3D bioprinted scaffold released higher levels of nerve growth factor (NGF) than 2D culture group. Further, the mRNA levels of NGF, brain-derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), and platelet-derived growth factor (PDGF) expressed on day 4 by Schwann cells were higher in the 3D bioprinted scaffold culture than in 2D culture. After 4 weeks of implantation, the cell-containing scaffold still showed partial lattice structure and positive S-100β immunofluorescence. These results indicated that the 3D bioprinted gelatin-sodium alginate/Schwann-cell composite scaffold improved cell adhesion and related factor expression. This 3D bioprinted composite scaffold showed good biocompatibility and could be a promising candidate in neural tissue engineering in the future.
周围神经损伤常导致不同程度的感觉和运动功能丧失。目前，“金标准”自体神经移植的修复效果并不理想。组织工程具有组织操作、再生和生长的潜力，但实现个性化和精确性仍然是一个挑战。在这项研究中，我们使用 3D 生物打印技术构建了一种由含有大鼠雪旺细胞的明胶/海藻酸钠水凝胶组成的神经支架。打印后第 1 天，雪旺细胞的存活率为 91.87±0.55%。细胞可以在水凝胶中培养 7 天，并且很好地附着在支架的表面上。在第 4 天和第 7 天，3D 生物打印支架释放的神经生长因子 (NGF) 水平高于 2D 培养组。此外，在第 4 天，3D 生物打印支架培养的雪旺细胞表达的神经生长因子 (NGF)、脑源性神经营养因子 (BDNF)、胶质源性神经营养因子 (GDNF) 和血小板源性生长因子 (PDGF) 的 mRNA 水平均高于 2D 培养组。植入 4 周后，含有细胞的支架仍显示出部分格子结构和 S-100β 免疫荧光阳性。这些结果表明，3D 生物打印的明胶-海藻酸钠/雪旺细胞复合支架改善了细胞黏附及相关因子的表达。这种 3D 生物打印的复合支架具有良好的生物相容性，有望成为未来神经组织工程的候选材料。
