长链非编码 RNA 2310043L19Rik 通过海绵吸附 miR-125a-5p 抑制成肌细胞分化并促进增殖。

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p.

机构信息

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.

出版信息

Aging (Albany NY). 2020 Mar 31;12(7):5625-5639. doi: 10.18632/aging.102905.

Although many long non-coding RNAs (lncRNAs) have been identified in muscle, some of their physiological functions and regulatory mechanisms remain elusive. Here we report the functional identification and characterization of a novel lncRNA 2310043L19Rik (lnc-231), which is highly expressed in muscle. The expression level of lnc-231 in skeletal muscle of young mice is higher than that in aged mice. Functional analysis showed that overexpression of lnc-231 restrained differentiation and promoted proliferation of myoblast, while inhibition of lnc-231 revealed completely opposite effects . RNA molecules of lnc-231 acted mechanistically as competing endogenous RNAs (ceRNA) to target miR-125a-5p, whereas miR-125a-5p binds to the 3'-UTR of E2F3 mRNA to inhibit its function. Collectively, lncRNA 2310043L19Rik promotes proliferation and inhibits differentiation of myoblast cells by attenuating the function of miR-125a-5p.

虽然在肌肉中已经鉴定出许多长链非编码 RNA（lncRNA），但其一些生理功能和调控机制仍不清楚。在这里，我们报告了一种新型 lncRNA 2310043L19Rik（lnc-231）的功能鉴定和表征，该 lncRNA 在肌肉中高度表达。年轻小鼠骨骼肌中 lnc-231 的表达水平高于老年小鼠。功能分析表明，lnc-231 的过表达抑制了成肌细胞的分化并促进了其增殖，而 lnc-231 的抑制则产生了完全相反的效果。lnc-231 的 RNA 分子作为竞争性内源 RNA（ceRNA）发挥作用，靶向 miR-125a-5p，而 miR-125a-5p 结合到 E2F3 mRNA 的 3'-UTR 上以抑制其功能。总之，lncRNA 2310043L19Rik 通过减弱 miR-125a-5p 的功能促进成肌细胞的增殖并抑制其分化。