University Lille, CNRS, Inserm, CHU Lille, Centre Oscar Lambret, UMR 9020 - UMR 1277 - Canther - Cancer Heterogeneity, Plasticity and Resistance to Therapies, F-59000 Lille, France.
Genes (Basel). 2020 Mar 27;11(4):362. doi: 10.3390/genes11040362.
The Polycomb Repressive Complex 1 (PRC1) is a chromatin-associated protein complex involved in transcriptional repression of hundreds of genes controlling development and differentiation processes, but also involved in cancer and stem cell biology. Within the canonical PRC1, members of Pc/CBX protein family are responsible for the targeting of the complex to specific gene loci. In mammals, the Pc/CBX protein family is composed of five members generating, through mutual exclusion, different PRC1 complexes with potentially distinct cellular functions. Here, we performed a global analysis of the gene family in 68 teleost species and traced the distribution of the genes through teleost evolution in six fish super-orders. We showed that after the teleost-specific whole genome duplication, , and are retained as pairs of ohnologues. In contrast, and are present as pairs of ohnologues in the genome of several teleost clades but as singletons in others. Furthermore, since zebrafish is a widely used vertebrate model for studying development, we report on the expression of the family members during zebrafish development and in adult tissues. We showed that all genes are ubiquitously expressed with some variations during early development.
多梳抑制复合物 1（PRC1）是一种与染色质相关的蛋白复合物，参与数百个基因的转录抑制，这些基因控制着发育和分化过程，但也参与癌症和干细胞生物学。在典型的 PRC1 中，Pc/CBX 蛋白家族的成员负责将复合物靶向特定的基因座。在哺乳动物中，Pc/CBX 蛋白家族由五个成员组成，通过相互排斥作用，生成具有潜在不同细胞功能的不同 PRC1 复合物。在这里，我们对 68 种硬骨鱼类中的 基因家族进行了全面分析，并通过六个鱼类超目在硬骨鱼类进化过程中追踪了 基因的分布。我们表明，在硬骨鱼类特异性全基因组复制之后， 、 和 作为同源基因对保留下来。相比之下， 和 在一些硬骨鱼类类群的基因组中作为同源基因对存在，但在其他类群中则作为单拷贝存在。此外，由于斑马鱼是研究发育的广泛使用的脊椎动物模型，我们报告了 家族成员在斑马鱼发育和成年组织中的表达情况。我们表明，所有 基因都广泛表达，在早期发育过程中有一些变化。
