Treatment efficacy of , , , and extracts in -induced rabbit dermatitis model.

BACKGROUND AND AIM

Dermatitis is a soft-tissue infection caused by . The recurrence of inflammatory skin is linked to clinical manifestations. Anti-inflammatory cytokines, which are essential for tissue damage, are released by bacteria through skin tissues. Oxidative stress causes inflammatory cells to necrotize and reduces their antioxidant profile, resulting in toxic damage to surrounding tissues. Although studies on the antibacterial effects of Lindl., L., L., and (Burm.). Bacterial infection of have been conducted, most of these studies have been and were not related to the rabbit model. In addition, anti-inflammatory and antioxidant studies need to be evaluated. Thus, this study aims to compare the antibacterial, anti-inflammatory, and antioxidant properties of four local herbs with a standard antibiotic in -induced rabbit dermatitis model.

MATERIALS AND METHODS

The skin of New Zealand white rabbits were artificially wounded using a sterile blade and then infected with . The rabbits were divided into seven groups, each with three rabbits (Total 21 rabbits): The first group was the no infection group (no infection and no treatment with scarification), the second group was the no treatment group ( infection of the wound but no treatment), and the other five treated groups were , and bacitracin cream, all of which involved wound infection and treatments. The treatment lasted for 7 days. The antibacterial, anti-inflammatory, and antioxidant properties after treatment were measured.

RESULTS

The efficacy of , , , and was similar to that of an antioxidant and free radical scavenging property. The bacterial infection process gradually reduced the activities of antioxidant systems (i.e., enzymatic levels and gene expressions) and total glutathione. However, the activities of the antioxidant system were steadily increased when treated with herbal extracts. During bacterial invasion of the skin, the concentration of thiobarbituric acid reactive molecules, the level of lipid peroxidation, and the expression of anti-inflammatory cytokine genes were increased. All these were decreased when herbal extracts were used to treat the lesion.

CONCLUSION

It can be concluded that , and . paniculate extract have antibacterial, anti-inflammatory, and antioxidant properties and are effective antibacterial agents. is the most effective herbal extract for antidermatitis and has the potential to be used as an alternative topical treatment.