Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Turkey.
Genetic and Bioengineering Department, Faculty of Engineering and Architecture, Ahi Evran University, Kirsehir, Turkey.
J Sci Food Agric. 2020 Jun;100(8):3525-3535. doi: 10.1002/jsfa.10400. Epub 2020 Apr 18.
Propolis exhibits therapeutic properties due to the presence of phenolic acids, esters, and flavonoids. The scope of this study was to develop a nano-vesicular formulation and establish a three-dimensional (3D) spheroid model in which lung cancer is recapitulated.
Niosome vesicles doped with galangin-rich propolis extract were synthesized by the ether injection method using a cholesterol : surfactant mass ratio of 1 : 3 at 40 °C for 1 h. Formulated niosomes were administered to 3D lung cancer spheroid model and the cytotoxicity was compared with that of a two-dimensional (2D) setting. The galangin content was determined as 86 μg mg propolis extract by ultra-performance liquid chromatography (UPLC). The particle size of loaded niosome was 151 ± 2.84 nm with a polydispersity index (PDI) of about 0.232, and an encapsulation efficiency of 70% was achieved.
The decrease in cell viability and the scattering in the 3D spheroids of A549 lung cancer cells treated with propolis-loaded niosomes were notable, indicating a profound cytotoxic effect and suggesting that they can be utilized as an effective nano-vesicle. © 2020 Society of Chemical Industry.
蜂胶因含有酚酸、酯和类黄酮而具有治疗特性。本研究的目的是开发一种纳米囊泡制剂，并建立一种能够重现肺癌的三维（3D）球体模型。
采用乙醚注入法，以胆固醇：表面活性剂质量比为 1：3，在 40°C 下反应 1 小时，合成了富含姜黄素的蜂胶提取物掺杂的脂质体囊泡。将制备的脂质体囊泡给药于 3D 肺癌球体模型，并将其细胞毒性与二维（2D）环境进行比较。通过超高效液相色谱（UPLC）测定姜黄素的含量为 86μg mg 蜂胶提取物。载药脂质体的粒径为 151±2.84nm，多分散指数（PDI）约为 0.232，包封效率为 70%。
用载有蜂胶的脂质体处理 A549 肺癌细胞的 3D 球体中的细胞活力下降和散射明显，表明其具有显著的细胞毒性作用，可作为有效的纳米囊泡使用。 © 2020 化学工业协会。
