Department of Pharmaceutical Technology, Department of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, Ege University, İzmir, Turkey.
Department of Biomedical Technologies, Graduate Faculty of Natural and Applied Science, Ege University, İzmir, Turkey.
Nutr Cancer. 2022;74(1):265-277. doi: 10.1080/01635581.2021.1876889. Epub 2021 Feb 16.
Propolis is a candidate for cancer treatment with its activity against different tumor cells and, has a wide spectrum of biological and pharmacological activities due to the diversity of its components. In this study, antitumorigenic activities of ethanol extract of propolis (EEP) and ethanol extract of propolis loaded niosome (PLN) were compared using 2D and 3D cell culture. Niosome formulations were prepared by thin film hydration technique. Cell viability of EEP and PLN was analyzed on MCF7, A549, MDA-MB-231, SK-MEL, SK-BR-3, DU145 and L-929 cell lines using MTT assay. L929, MCF7 and A549 cells were cultured using the 3D petri dish technique and their spherical forms were obtained after 142 h. After 24 h, PLN and EEP application, cell viability analysis was performed on 3D cultures with WST assay. As a result, niosome formulations containing EEP showed higher activity than ethanol extract of propolis in cancer cells. While a slow decrease was observed in cell viability in EEP treated cancer cells, it was observed that the percentage viability rates decreased in a shorter time in PLN treated cancer cells. Also, PLN can be used as an anticancer activity drug such as Doxorubicin, but this is not the case for EEP.
蜂胶是一种有潜力的癌症治疗药物，对不同的肿瘤细胞具有活性，由于其成分的多样性，具有广泛的生物和药理活性。在这项研究中，使用 2D 和 3D 细胞培养比较了蜂胶乙醇提取物 (EEP) 和载有蜂胶乙醇提取物的尼森体 (PLN) 的抗肿瘤活性。尼森体制剂通过薄膜水化技术制备。MTT 分析测定 EEP 和 PLN 对 MCF7、A549、MDA-MB-231、SK-MEL、SK-BR-3、DU145 和 L-929 细胞系的细胞活力。使用 3D 培养皿技术培养 L929、MCF7 和 A549 细胞，142 小时后获得它们的球形形式。24 小时后，用 WST 测定法在 3D 培养物上进行 PLN 和 EEP 应用后的细胞活力分析。结果表明，含 EEP 的尼森体制剂在癌细胞中的活性高于蜂胶乙醇提取物。虽然在 EEP 处理的癌细胞中观察到细胞活力缓慢下降，但在 PLN 处理的癌细胞中，细胞活力的百分比在更短的时间内下降。此外，PLN 可以像多柔比星一样用作抗癌活性药物，但 EEP 并非如此。
