Laboratory of Sustainable Animal Production and Biodiversity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Biological Function and Engineering, Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Fukuoka 808-0196, Japan.
Waste Manag. 2020 Apr 1;106:240-249. doi: 10.1016/j.wasman.2020.03.029. Epub 2020 Mar 30.
Biochar has proven to be a feasible additive for mitigating nitrogen loss during the composting process. This study aims to evaluate the influence of biochar addition on bacterial community and physicochemical properties changes, including ammonium (NH), nitrite (NO) and nitrate (NO) contents during the composting of poultry manure. The composting was carried out by adding 20% (w/w) of biochar into the mixture of poultry manure and rice straw with a ratio of 2:1, and the same treatment without biochar was prepared as a control. The finished product of control compost recorded the high contents of NO and NO (366 mg/kg and 600 mg/kg) with reduced the total NH content to 10 mg/kg. Meanwhile, biochar compost recorded a higher amount of total NH content (110 mg/kg) with low NO and NO (161 mg/kg and 137 mg/kg) content in the final composting material. The principal component analysis showed that the dynamics of dominant genera related to Halomonas, Pusillimonas, and Pseudofulvimonas, all of which were known as nitrifying and denitrifying bacteria, was significantly correlated with the dynamic of NO and NO content throughout the composting process. The genera related to Pusillimonas, and Pseudofulvimonas appeared as the dominant communities as the NO and NO increased. In contrast, as the NO and NO concentration decreased, the Halomonas genus were notably enriched in biochar compost. This study revealed the bacterial community shifts corresponded with the change of physicochemical properties, which provides essential information for a better understanding of monitoring and improving the composting process.
生物炭已被证明是一种可行的添加剂，可减少堆肥过程中的氮损失。本研究旨在评估添加生物炭对细菌群落和理化性质变化的影响，包括在鸡粪堆肥过程中铵（NH）、亚硝酸盐（NO）和硝酸盐（NO）的含量。堆肥是通过将 20%（w/w）的生物炭添加到鸡粪和稻草的混合物中（比例为 2:1）来进行的，并用相同的不含生物炭的处理作为对照。对照堆肥的成品记录了高含量的 NO 和 NO（366 mg/kg 和 600 mg/kg），同时将总 NH 含量降低到 10 mg/kg。与此同时，生物炭堆肥记录了更高的总 NH 含量（110 mg/kg），而最终堆肥材料中的 NO 和 NO 含量较低（161 mg/kg 和 137 mg/kg）。主成分分析表明，与硝化和反硝化细菌有关的优势属，如 Halomonas、Pusillimonas 和 Pseudofulvimonas 的动态与整个堆肥过程中 NO 和 NO 含量的动态显著相关。与 Pusillimonas 和 Pseudofulvimonas 相关的属随着 NO 和 NO 的增加而成为优势群落。相比之下，随着 NO 和 NO 浓度的降低，Halomonas 属在生物炭堆肥中明显富集。本研究揭示了细菌群落的变化与理化性质的变化相对应，为更好地理解监测和改善堆肥过程提供了重要信息。
