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巨噬细胞衍生的细胞外囊泡促进毛发生长。

Macrophage-Derived Extracellular Vesicle Promotes Hair Growth.

机构信息

Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, Korea.

Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu-41944, Korea.

出版信息

Cells. 2020 Apr 1;9(4):856. doi: 10.3390/cells9040856.

DOI:10.3390/cells9040856
PMID:32244824
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7226775/
Abstract

Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.

摘要

脱发是一种常见的医学问题，影响男性和女性。真皮乳头（DP）细胞是脱发患者头发再生潜在细胞的最终储备库。在这里，我们分析了巨噬细胞衍生的 Wnts（3a 和 7b）和巨噬细胞细胞外囊泡（MAC-EVs）在促进头发生长中的作用。我们研究了 MAC-EVs 存在或不存在时人-DP 细胞的增殖、迁移和生长因子表达。此外，我们还测试了 MAC-EV 处理对小鼠模型和人毛囊中毛发生长的影响。Western blot 和流式细胞术的数据表明，MAC-EVs 富含 Wnt3a 和 Wnt7b，超过 95%与它们的膜相关。结果表明，MAC-EVs 中的 Wnt 蛋白激活了 Wnt/β-连环蛋白信号通路，导致转录因子（）的激活。MAC-EVs 显著增强 DP 细胞的增殖、迁移和毛发生长诱导标志物的水平。此外，MAC-EVs 磷酸化 AKT 并增加生存蛋白 Bcl-2 的水平。用 MAC-EVs 处理的 DP 细胞显示出血管内皮生长因子（VEGF）和角质细胞生长因子（KGF）的表达增加。用 MAC-EVs 处理 Balb/c 小鼠可在体内促进毛囊（HF）生长，并在短时间内增加人 HF 中的毛干大小。我们的研究结果表明，MAC-EV 治疗可作为治疗脱发的一种有前途的新型生长期诱导剂在临床上应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/530730f76488/cells-09-00856-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/8e29e8bbca3b/cells-09-00856-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/fdbdd35a9f71/cells-09-00856-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/1f5010bc3177/cells-09-00856-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/c7c756b5848e/cells-09-00856-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/8648c6a55431/cells-09-00856-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/748d1923ead6/cells-09-00856-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/6d28dc5f2005/cells-09-00856-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/530730f76488/cells-09-00856-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/8e29e8bbca3b/cells-09-00856-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/fdbdd35a9f71/cells-09-00856-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/1f5010bc3177/cells-09-00856-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/c7c756b5848e/cells-09-00856-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/8648c6a55431/cells-09-00856-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/748d1923ead6/cells-09-00856-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/6d28dc5f2005/cells-09-00856-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b85/7226775/530730f76488/cells-09-00856-g008.jpg

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