Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Korea.
BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Korea.
FEBS Lett. 2021 Apr;595(7):942-953. doi: 10.1002/1873-3468.14050. Epub 2021 Feb 24.
Hair loss is a prevalent medical condition affecting both genders. In this study, we investigate the effects of a specific class of extracellular vesicles (EVs), namely human normal fibroblast-derived EVs (hFB-EVs), on human dermal papilla (DP) and outer root sheath (ORS) cells and examine the molecular mechanisms responsible for hair growth in hair follicles (HFs). We find that Wnt3a, which maintains the hair-generating activity of DP cells, is enriched and more strongly associated with hFB-EVs than with fibroblasts. Furthermore, hFB-EV-associated Wnt3a mediated receptor activation in cultured DP cells, leading to an increase in β-catenin in the cytoplasm and its translocation into the nucleus, thereby elevating expression of the target genes Axin2 and Lef1. Additionally, hFB-EVs promoted the migration, proliferation, and differentiation of ORS cells and elongation of the hair shaft in human HFs. These findings revealed a novel mechanism by which hFB-EVs influence hair growth.
脱发是一种普遍存在的医学病症，影响男女两性。在这项研究中，我们研究了一类特定的细胞外囊泡（EVs），即人正常成纤维细胞衍生的 EVs（hFB-EVs），对人真皮乳头（DP）和外根鞘（ORS）细胞的影响，并研究了毛发生长过程中毛囊（HFs）中负责的分子机制。我们发现，Wnt3a 能维持 DP 细胞的毛发生成活性，它在 hFB-EVs 中的富集程度更高，与 hFB-EVs 的结合也更强。此外，hFB-EV 相关的 Wnt3a 在培养的 DP 细胞中介导受体激活，导致细胞质中的 β-连环蛋白增加，并向细胞核内转移，从而提高靶基因 Axin2 和 Lef1 的表达。此外，hFB-EVs 促进了 ORS 细胞的迁移、增殖和分化，并使人类 HFs 中的毛干伸长。这些发现揭示了 hFB-EVs 影响毛发生长的新机制。
