Cruciani Sara, Garroni Giuseppe, Ginesu Giorgio Carlo, Fadda Angela, Ventura Carlo, Maioli Margherita
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
General Surgery Unit 2 "Clinica Chirurgica", Department of Medical, Surgical and Experimental Sciences, University of Sassari, Viale San Pietro 8, 07100 Sassari, Italy.
Biology (Basel). 2020 Mar 20;9(3):57. doi: 10.3390/biology9030057.
Cellular senescence plays a role in the onset of age-related pathologies and in the loss of tissue homeostasis. Natural compounds of food or plants exert an important antioxidant activity, counteracting the formation of harmful free radicals. In the presence of an intense stressing event, cells activate specific responses to counteract senescence or cell death. In the present paper, we aimed at evaluating the levels of expression of specific markers of senescence, in order to demonstrate that extracts from . can prevent premature senescence in ADSCs exposed to oxidative stress. Cells were cultured in the presence of extracts for 12-24 and 48 h and then incubated with HO to induce senescence. We then evaluated the expression of senescence-related markers p16, p19, p21, p53, TERT, c-Myc, and the senescence-associated β-Galactoidase activity. Our results showed that pre-treatment with extracts protects cells from premature senescence, by regulating the cell cycle, and inducing the expression of TERT and c-Myc. These findings suggest a potential application of these natural compounds in the prevention and treatment of various diseases, counteracting premature senescence and preserving tissue functions.
细胞衰老在与年龄相关的病理过程的发生以及组织稳态的丧失中起作用。食物或植物中的天然化合物具有重要的抗氧化活性，可对抗有害自由基的形成。在强烈应激事件存在的情况下，细胞会激活特定反应以对抗衰老或细胞死亡。在本论文中，我们旨在评估衰老特异性标志物的表达水平，以证明来自……的提取物可以预防暴露于氧化应激的脂肪干细胞过早衰老。将细胞在提取物存在的情况下培养12 - 24小时和48小时，然后用H₂O₂孵育以诱导衰老。然后我们评估了衰老相关标志物p16、p19、p21、p53、端粒酶逆转录酶（TERT）、c-Myc以及衰老相关β-半乳糖苷酶活性的表达。我们的结果表明，用提取物预处理可通过调节细胞周期并诱导TERT和c-Myc的表达来保护细胞免于过早衰老。这些发现表明这些天然化合物在预防和治疗各种疾病方面具有潜在应用，可对抗过早衰老并维持组织功能。
