Cruciani Sara, Trenta Margherita, Rassu Giovanna, Garroni Giuseppe, Petretto Giacomo Luigi, Ventura Carlo, Maioli Margherita, Pintore Giorgio
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
Department of Chemistry and Pharmacy, University of Sassari, Via F. Muroni 23/b, 07100 Sassari, Italy.
Antioxidants (Basel). 2021 Feb 3;10(2):227. doi: 10.3390/antiox10020227.
Dermal fibroblasts are the main actor in many proteins' secretion, including collagen, preserving skin function. Free radicals are involved in skin aging and damages involving different cellular components. The imbalance between reactive oxygen species (ROS) amount and natural antioxidant enzymes negatively affects skin homeostasis. Natural compounds have recently emerged as a potential anti-aging tool in tissue regeneration. In the present paper we evaluated the antioxidant activity of white and red wines, considering their probable use, as raw materials, for the formulation of cosmetic products with anti-aging properties. We studied a method that would allow the removal of the alcoholic fraction of wines and determined their composition by LC-MS analysis. We then tested the possible cytotoxic effects of red and white wines on fibroblasts by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, and their antioxidant activity by the catalase activity test in stressing conditions. Finally, we evaluated their anti-aging potential through the β-galactosidase colorimetric assay. Our results showed that wine extracts exhibit a remarkable antioxidant and anti-aging activity, especially on cells exposed to a marked stressful event. These properties could suggest their possible application as cosmetical products for skin regeneration.
真皮成纤维细胞是包括胶原蛋白在内的多种蛋白质分泌的主要参与者，维持着皮肤功能。自由基参与皮肤衰老以及涉及不同细胞成分的损伤过程。活性氧（ROS）量与天然抗氧化酶之间的失衡会对皮肤稳态产生负面影响。天然化合物最近已成为组织再生中一种潜在的抗衰老工具。在本文中，我们评估了白葡萄酒和红葡萄酒的抗氧化活性，考虑到它们作为原料可能用于配制具有抗衰老特性的化妆品。我们研究了一种能够去除葡萄酒酒精成分的方法，并通过液相色谱 - 质谱（LC - MS）分析确定其成分。然后，我们通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）法测试了红葡萄酒和白葡萄酒对成纤维细胞可能的细胞毒性作用，并通过在应激条件下的过氧化氢酶活性测试来检测它们的抗氧化活性。最后，我们通过β - 半乳糖苷酶比色法评估了它们的抗衰老潜力。我们的结果表明，葡萄酒提取物具有显著的抗氧化和抗衰老活性，尤其是对暴露于明显应激事件的细胞。这些特性表明它们可能作为用于皮肤再生的化妆品应用。
