Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing 100190, China.
Nutrients. 2020 Mar 20;12(3):826. doi: 10.3390/nu12030826.
We aimed to identify if maternal and infant factors were associated with neutral human milk oligosaccharides (HMOs) variability and examined the associations between HMOs concentration and infant growth and disease status in healthy Chinese mothers over a 6-month lactation period. We recruited mothers and their full-term infants as our subjects. At 1-5 days, 8-14 days, 4 weeks, and 6 months postpartum, all participants were interviewed to collect breast milk samples, obtain follow-up data and measure infant length and weight at their local hospital. A total of 23 neutral HMOs were analyzed by high performance liquid chromatography (HPLC)- mass spectrometer (MS). Secretor and Lewis phenotype were determined by the concentration of 2'-fucosyllactose (2'-FL) and Lacto-N-fucopentaose (LNFP)-II. The associations between maternal and infant factors with HMOs concentrations were investigated. A total of 464 human breast milk samples were collected from 116 mothers at four different time points. In total, 76.7% mothers were found to be Secretor and Lewis positive phenotype (Se+Le+), 17.2% were Se-Le+, 4.3% were Se+Le-, and 1.7% were Se-Le-. Several individual HMOs, including 2'-FL, Lactodifucotetraose (LDFT), LNFP-I were determined by Secretor phenotype. Most individual HMOs decreased at the later stage of lactation, except 3'-FL. We suggest that Secretor phenotype and lactation stage could influence most of the neutral HMOs. Concentrations of specific HMOs may be associated with maternal age, allergic history, pre-pregnancy body mass index (BMI), parity, delivery mode, infant gestational age and gender.
我们旨在确定母婴因素是否与中性人乳寡糖（HMOs）的变异性相关，并在健康的中国母亲哺乳 6 个月期间，检查 HMOs 浓度与婴儿生长和疾病状况之间的关联。我们招募了母亲及其足月婴儿作为研究对象。在产后 1-5 天、8-14 天、4 周和 6 个月时，所有参与者都接受了采访，以收集母乳样本、获取随访数据，并在当地医院测量婴儿的身长和体重。采用高效液相色谱（HPLC）-质谱（MS）法分析了 23 种中性 HMOs。通过 2'-岩藻糖基乳糖（2'-FL）和乳-N-岩藻五糖（LNFP）-II 的浓度确定分泌型和 Lewis 表型。调查了母婴因素与 HMOs 浓度之间的关系。共从 116 位母亲的 4 个不同时间点采集了 464 个人乳样本。总共有 76.7%的母亲被发现是分泌型和 Lewis 阳性表型（Se+Le+），17.2%是 Se-Le+，4.3%是 Se+Le-，1.7%是 Se-Le-。几种个体 HMOs，包括 2'-FL、乳二岩藻四糖（LDFT）、LNFP-I，是通过分泌型表型确定的。除了 3'-FL，大多数个体 HMOs 在哺乳后期下降。我们认为分泌型表型和泌乳阶段可能会影响大多数中性 HMOs。特定 HMOs 的浓度可能与母亲年龄、过敏史、孕前体重指数（BMI）、产次、分娩方式、婴儿胎龄和性别有关。
