母乳生物活性物质的膳食调节与母体FUT2分泌型表型相关：类胡萝卜素和多酚代谢物的探索性分析

Dietary modulation of human milk bioactives is associated with maternal FUT2 secretor phenotype: an exploratory analysis of carotenoids and polyphenol metabolites.

作者信息

Fiecke Chelsey, Crimmins Meghan, Hameed Ahsan, Sims Clark, Williams D Keith, Bode Lars, Martinez Audrey, Andres Aline, Ferruzzi Mario G

机构信息

Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States.

Department of Pediatrics, Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CORE), and the Human Milk Institute (HMI), University of California, San Diego, La Jolla, CA, United States.

出版信息

Front Nutr. 2024 Oct 9;11:1463969. doi: 10.3389/fnut.2024.1463969. eCollection 2024.

DOI:10.3389/fnut.2024.1463969

PMID:39444568

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11496265/

Abstract

INTRODUCTION

Maternal diet modifies profiles of human milk oligosaccharides (HMOs), carotenoids, and polyphenols in human milk (HM). However, substantial variability in profiles exists between women, highlighting the complexity of non-dietary factors modulating these profiles. The objective of this study was to carry out a secondary analysis exploring the effect of maternal diet on HM carotenoids and polyphenols and relationships between dietary modulation of HM bioactives (carotenoids, polyphenols, and oligosaccharides) and maternal α1,2-fucosyltransferase 2 (FUT2) secretor phenotype.

METHODS

In this pilot study, 16 exclusively breastfeeding women with obesity were enrolled between 4 and 5 months postpartum. The women were provided a 4-week meal plan consistent with the 2020 Dietary Guidelines for Americans (DGA). HM was collected for 24 h at baseline and post-intervention. Maternal FUT2 secretor phenotype was determined by 2'-fucosyllactose concentration in HM (non-secretor: < 100 nmol/ml; secretor: ≥100 nmol/ml). Concentrations of carotenoids and HMOs were determined by LC and polyphenol metabolites by UPLC-MS/MS.

RESULTS

Thirteen women completed the study (6 secretors, 7 non-secretors). The change in HM concentrations of the HMOs lacto-N-tetraose (LNT, = 0.007), lacto-N-fucopentaose II (LNFP II, = 0.02), difucosyllacto-N-tetraose (DFLNT, = 0.003), and disialyllacto-N-tetraose (DSLNT, = 0.003) and polyphenol metabolites 4-hydroxybenzoic acid (4-HBA, = 0.08) and ferulic acid ( = 0.02) over the intervention time frame was differentially associated with maternal secretor status. 4-HBA and ferulic acid positively correlated with HMOs LNT and DSLNT ( = 0.82-0.90, = 0.03-0.06) for secretors but not for non-secretors. Only secretors demonstrated a negative correlation between 4-HBA and DFLNT ( = -0.94, = 0.001).

DISCUSSION

The influence of maternal diet on composition of HMOs and polyphenol metabolites in HM differs based on maternal secretor status. Consideration of non-dietary factors is needed to evaluate differences in response of HM bioactives to dietary modulation.

摘要

引言

母亲的饮食会改变母乳中母乳低聚糖（HMOs）、类胡萝卜素和多酚的成分。然而，不同女性之间这些成分存在很大差异，这凸显了调节这些成分的非饮食因素的复杂性。本研究的目的是进行二次分析，探讨母亲饮食对母乳中类胡萝卜素和多酚的影响，以及母乳生物活性物质（类胡萝卜素、多酚和低聚糖）的饮食调节与母亲α1,2-岩藻糖基转移酶2（FUT2）分泌型之间的关系。

方法

在这项试点研究中，16名产后4至5个月的肥胖纯母乳喂养女性被纳入研究。为这些女性提供了一份符合《2020年美国膳食指南》（DGA）的4周饮食计划。在基线和干预后收集24小时的母乳。通过母乳中2'-岩藻糖乳糖浓度确定母亲的FUT2分泌型（非分泌型：<100 nmol/ml；分泌型：≥100 nmol/ml）。通过液相色谱法测定类胡萝卜素和HMOs的浓度，通过超高效液相色谱-串联质谱法测定多酚代谢物的浓度。

结果

13名女性完成了研究（6名分泌型，7名非分泌型）。在干预时间段内，母乳中HMOs的乳糖-N-四糖（LNT，=0.007）、乳糖-N-岩藻五糖II（LNFP II，=0.02）、二岩藻糖基乳糖-N-四糖（DFLNT，=0.003）和二唾液酸乳糖-N-四糖（DSLNT，=0.003）以及多酚代谢物4-羟基苯甲酸（4-HBA，=0.08）和阿魏酸（=0.02）的变化与母亲的分泌型状态存在差异关联。对于分泌型女性，4-HBA和阿魏酸与HMOs的LNT和DSLNT呈正相关（=0.82-0.90，=0.03-0.06），而非分泌型女性则不然。只有分泌型女性表现出4-HBA与DFLNT之间呈负相关（= -0.94，=0.001）。