Fusco Roberta, Siracusa Rosalba, Peritore Alesso Filippo, Gugliandolo Enrico, Genovese Tiziana, D'Amico Ramona, Cordaro Marika, Crupi Rosalia, Mandalari Giuseppina, Impellizzeri Daniela, Cuzzocrea Salvatore, Di Paola Rosanna
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Department of Biomedical, Dental and Morphological and Functional Imaging University of Messina, Via Consolare Valeria, 98125 Messina, Italy.
Antioxidants (Basel). 2020 Jun 10;9(6):511. doi: 10.3390/antiox9060511.
Osteoarthritis is a progressive joint disease characterized by the activation of different molecular mediators, including proinflammatory cytokines, reactive oxygen species, metalloproteinases and nociceptive mediators. L. is a medicinal plant with anti-oxidative and anti-inflammatory properties. In this study we evaluate the effects of cashew nuts (from L.) oral administration on an experimental model of painful degenerative joint disease. Monosodium iodoacetate (MIA) was intraarticularly injected, and cashew nuts were orally administered three times per week for 21 days, starting the third day after MIA injection. Nociception was evaluated by a Von Frey filament test, and motor function by walking track analysis at 3, 7, 14 and 21 days after osteoarthritis. Histological and biochemical alteration were examined at the end of the experiment. Cashew nuts administration reduced pain-like behavior and showed antioxidant activities, restoring biochemical serum parameters: glutathione (GSH), catalase (CAT) levels, glutathione peroxidase (GPx) activity and lipid peroxidation. Moreover, cashew nuts ameliorated radiographic and histological alteration, resulting in decreased cartilage degradation, pro-inflammatory cytokines and metalloproteinases levels and mast cells recruitment. Our results demonstrated that the oral assumption of cashew nuts counteracts the inflammatory and oxidative process involved in osteoarthritis.
骨关节炎是一种进行性关节疾病，其特征是多种分子介质被激活，包括促炎细胞因子、活性氧、金属蛋白酶和伤害性介质。腰果树是一种具有抗氧化和抗炎特性的药用植物。在本研究中，我们评估了口服腰果树（来自腰果树）对疼痛性退行性关节疾病实验模型的影响。关节内注射碘乙酸钠（MIA），从MIA注射后的第三天开始，每周口服腰果树三次，持续21天。在骨关节炎发生后的第3、7、14和21天，通过von Frey细丝试验评估伤害感受，通过行走轨迹分析评估运动功能。在实验结束时检查组织学和生化改变。口服腰果树可减轻疼痛样行为并显示出抗氧化活性，恢复生化血清参数：谷胱甘肽（GSH）、过氧化氢酶（CAT）水平、谷胱甘肽过氧化物酶（GPx）活性和脂质过氧化。此外，腰果树改善了影像学和组织学改变，导致软骨降解、促炎细胞因子和金属蛋白酶水平降低以及肥大细胞募集减少。我们的结果表明，口服腰果树可对抗骨关节炎中涉及的炎症和氧化过程。
