Biotechnology, Department of Chemistry, Lund University, PO Box 124, SE-221 00, Lund, Sweden.
Food Technology, Engineering and Nutrition, Lund University, PO Box 124, SE-221 00, Lund, Sweden.
Sci Rep. 2020 Apr 3;10(1):5853. doi: 10.1038/s41598-020-62610-x.
The importance of the gut microbiota in human health has led to an increased interest to study probiotic bacteria. Fermented food is a source of already established probiotics, but it also offers an opportunity to discover new taxa. Four strains of Weissella sp. isolated from Indian fermented food have been genome sequenced and classified into the species W. cibaria based on whole-genome phylogeny. The genome of W. cibaria strain 92, known to utilise xylooligosaccharides and produce lactate and acetate, was analysed to identify genes for oligosaccharide utilisation. Clusters including genes involved in transportation, hydrolysis and metabolism of xylooligosaccharides, arabinooligosaccharides and β-glucosides were identified. Growth on arabinobiose and laminaribiose was detected. A 6-phospho-β-glucosidase clustered with a phosphotransferase system was found upregulated during growth on laminaribiose, indicating a mechanism for laminaribiose utilisation. The genome of W. cibaria strain 92 harbours genes for utilising the phosphoketolase pathway for the production of both acetate and lactate from pentose and hexose sugars but lacks two genes necessary for utilising the pentose phosphate pathway. The ability of W. cibaria strain 92 to utilise several types of oligosaccharides derived from dietary fibres, and produce lactate and acetate makes it interesting as a probiotic candidate for further evaluation.
肠道微生物群在人类健康中的重要性使得人们对益生菌的研究产生了浓厚的兴趣。发酵食品是已确定的益生菌的来源，但它也为发现新的分类群提供了机会。从印度发酵食品中分离出的 4 株魏斯氏菌已被进行全基因组测序，并根据全基因组系统发育分类为韦氏魏斯氏菌。W. cibaria 菌株 92 已知可利用木低聚糖并产生乳酸和乙酸，其基因组被分析以鉴定用于寡糖利用的基因。鉴定出了包括木低聚糖、阿拉伯低聚糖和β-葡萄糖苷水解和代谢相关基因的簇。检测到在阿拉伯二糖和纤维二糖上的生长。在利用纤维二糖生长过程中发现了与磷酸转移酶系统相关的 6-磷酸-β-葡萄糖苷酶簇，表明存在利用纤维二糖的机制。W. cibaria 菌株 92 的基因组具有利用磷酸酮醇途径从戊糖和己糖生产乙酸和乳酸的基因，但缺乏利用戊糖磷酸途径所需的两个基因。W. cibaria 菌株 92 能够利用几种来源于膳食纤维的低聚糖，并产生乳酸和乙酸，这使其成为进一步评估的有趣益生菌候选物。
