Li Shiao-Wen, Chen Yi-Sheng, Lee Yun-Shien, Yang Chih-Hsien, Srionnual Sirinat, Wu Hui-Chung, Chang Chuan-Hsiung
Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan.
Bioinformatics Program, Taiwan International Graduate Program, Institute of Information Science, Academia Sinica, Taipei, 11529, Taiwan.
Appl Microbiol Biotechnol. 2017 Feb;101(3):1227-1237. doi: 10.1007/s00253-016-8073-8. Epub 2017 Jan 5.
Weissella cibaria 110 was isolated from plaa-som, a Thai fermented fish product, and known to produce the weissellicin 110 bacteriocin. We carried out comprehensive comparative genomic analysis of W. cibaria 110 with four other non-bacteriocin-producing W. cibaria strains and identified potential antibiotic-resistant genes. We further identified a type III restriction-modification system, a TA system, and a bacteriocin gene cluster that are unique in W. cibaria 110. Genes related to bacteriocin biosynthesis are organized in clusters and are encoded with minimum genetic machinery consisting of structural cognate immunity genes, including ABC transporter and immunity protein. Finally, we predicted W. cibaria 110 to produce a class IId bacteriocin, weissellicin 110, which is 31 amino acids in length and contains a 21-amino-acid N-terminal leader peptide. This is the first bacteriocin-producing sequencing genome in W. cibaria, and we describe the difference between the bacteriocin-producing and non bacteriocin-producing strains from genome point of view.
魏斯氏菌110是从泰国发酵鱼制品普拉索姆中分离出来的，已知其能产生魏斯氏菌素110细菌素。我们对魏斯氏菌110与其他四种不产生细菌素的魏斯氏菌菌株进行了全面的比较基因组分析，并鉴定出潜在的抗生素抗性基因。我们进一步鉴定出了III型限制修饰系统、TA系统以及在魏斯氏菌110中独特的细菌素基因簇。与细菌素生物合成相关的基因成簇组织，并由包括ABC转运蛋白和免疫蛋白在内的结构同源免疫基因组成的最小遗传机制进行编码。最后，我们预测魏斯氏菌110会产生一种IId类细菌素——魏斯氏菌素110，其长度为31个氨基酸，包含一个21个氨基酸的N端前导肽。这是魏斯氏菌中首个产生细菌素的测序基因组，我们从基因组角度描述了产生细菌素的菌株与不产生细菌素的菌株之间的差异。
