Yin Chengbo, Wei Linpeng, Kose Kivanc, Glaser Adam K, Peterson Gary, Rajadhyaksha Milind, Liu Jonathan T C
Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.
Dermatology Service, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
J Biophotonics. 2020 Jun;13(6):e202000048. doi: 10.1002/jbio.202000048. Epub 2020 Apr 14.
Handheld and endoscopic optical-sectioning microscopes are being developed for noninvasive screening and intraoperative consultation. Imaging a large extent of tissue is often desired, but miniature in vivo microscopes tend to suffer from limited fields of view. To extend the imaging field during clinical use, we have developed a real-time video mosaicking method, which allows users to efficiently survey larger areas of tissue. Here, we modified a previous post-processing mosaicking method so that real-time mosaicking is possible at >30 frames/second when using a device that outputs images that are 400 × 400 pixels in size. Unlike other real-time mosaicking methods, our strategy can accommodate image rotations and deformations that often occur during clinical use of a handheld microscope. We perform a feasibility study to demonstrate that the use of real-time mosaicking is necessary to enable efficient sampling of a desired imaging field when using a handheld dual-axis confocal microscope.
手持式和内窥镜光学切片显微镜正在被开发用于无创筛查和术中会诊。通常希望对大面积组织进行成像，但微型体内显微镜往往视野有限。为了在临床使用中扩展成像视野，我们开发了一种实时视频拼接方法，该方法允许用户有效地观察更大面积的组织。在这里，我们改进了先前的后处理拼接方法，以便在使用输出尺寸为400×400像素图像的设备时，能够以每秒30帧以上的速度进行实时拼接。与其他实时拼接方法不同，我们的策略可以适应手持显微镜临床使用过程中经常出现的图像旋转和变形。我们进行了一项可行性研究，以证明使用实时拼接对于在使用手持双轴共聚焦显微镜时有效地对所需成像视野进行采样是必要的。
