Instituto de Investigación Lightbourn, A. C., Carretera Las Pampas Km. 2.5, 33980, Ciudad Jiménez, Chihuahua, México.
Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México.
Arch Virol. 2020 Jun;165(6):1485-1488. doi: 10.1007/s00705-020-04618-2. Epub 2020 Apr 4.
The GenBank database contains over 2580 complete genome sequences from bacteriophages. However, limited reports are available concerning phages can that lyse members of Pseudomonas syringae, although this is a widespread bacterial species that can infect almost 200 plant species. In the present study, we isolated and characterized a new Siphoviridae phage, named "Pseudomonas phage vB_PsyS_Phobos" (for brevity, referred to here as Phobos). To our knowledge, this is one of the first genome sequences reported for a phage with lytic activity against P. syringae pv. syringae. The genome of Phobos is dsDNA of 56,734 bp with a GC content of 63.3%, containing 65 ORFs. Genome analysis revealed that Phobos is a novel lytic phage with unique genomic features and low similarity to other phages, suggesting that Phobos represents a new phage genus. Genome sequencing did not reveal sequences with significant similarity to known virulence factors, antibiotic resistance genes, potential immunoreactive allergens, or lysogeny-related proteins, suggesting suggests that phage Phobos is strictly lytic. Therefore, Phobos may be suitable for formulation as a biocontrol agent against P. syringae pv. syringae.
GenBank 数据库中包含超过 2580 种完整的噬菌体基因组序列。然而，关于能够裂解丁香假单胞菌成员的噬菌体的报道有限，尽管丁香假单胞菌是一种广泛存在的细菌，可以感染近 200 种植物。在本研究中，我们分离并鉴定了一种新的肌尾噬菌体，命名为“Pseudomonas phage vB_PsyS_Phobos”（简称 Phobos）。据我们所知，这是第一个报道对丁香假单胞菌 pv. syringae 具有裂解活性的噬菌体的基因组序列之一。Phobos 的基因组是 56734bp 的双链 DNA，GC 含量为 63.3%，包含 65 个 ORFs。基因组分析表明，Phobos 是一种具有独特基因组特征和与其他噬菌体低相似性的新型裂解噬菌体，表明 Phobos 代表了一种新的噬菌体属。基因组测序未发现与已知毒力因子、抗生素耐药基因、潜在免疫反应性过敏原或溶原相关蛋白有显著相似性的序列，表明噬菌体 Phobos 严格是裂解性的。因此，Phobos 可能适合作为防治丁香假单胞菌 pv. syringae 的生物防治剂。
