Department of Dermatology and Center for Chronic Pruritus, University Hospital Münster, Münster, Germany.
Acta Derm Venereol. 2020 May 11;100(10):adv00131. doi: 10.2340/00015555-3476.
Isatis tinctoria L. (woad) has been used in medicine for centuries and has demonstrated anti-inflammatory effects. However, to date, no well-defined extracts with precise analysis of active substances have been developed. The aim of this study was to develop novel extracts of Isatis tinctoria L., and to characterize their active ingredients and anti-inflammatory properties. Various extracts of Isatis tinctoria L. were analysed for their active ingredients, and screened for anti-inflammatory effects using cyclooxygenase-2 activity assays. A petroleum ether extract was found to have the best effects, and was tested in a mouse model of acute allergic contact dermatitis. In the mouse model the petroleum ether extract resulted in significantly reduced ear swelling, oedema and inflammatory cell density. In mouse skin and human keratinocyte cultures, petroleum ether extract inhibited pro-inflammatory cytokine expression. Furthermore, human mast cell degranulation was significantly inhibited in LAD2 cell cultures. In conclusion, novel woad extracts were developed and shown to have anti-inflammatory properties in a contact hypersensitivity animal model and human keratinocytes. The production of such extracts and further characterization of their specific properties will enable determination of their potential dermatological effects in the treatment of inflamed and irritated skin.
菘蓝（菘蓝）在医学上已经使用了几个世纪，具有抗炎作用。然而，迄今为止，尚未开发出具有明确分析活性物质的精确定义提取物。本研究的目的是开发菘蓝的新型提取物，并对其有效成分和抗炎特性进行表征。分析了菘蓝的各种提取物中的有效成分，并通过环氧化酶-2 活性测定筛选其抗炎作用。发现石油醚提取物的效果最好，并在急性过敏性接触性皮炎的小鼠模型中进行了测试。在小鼠模型中，石油醚提取物导致耳肿胀、水肿和炎症细胞密度明显减少。在小鼠皮肤和人角质形成细胞培养物中，石油醚提取物抑制促炎细胞因子的表达。此外，人肥大细胞脱颗粒在 LAD2 细胞培养物中受到显著抑制。总之，开发了新型菘蓝提取物，并在接触性超敏反应动物模型和人角质形成细胞中显示出抗炎作用。此类提取物的生产及其特定性质的进一步表征将能够确定它们在治疗发炎和刺激性皮肤方面的潜在皮肤科作用。
