School of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Nutrition Research Center, School of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
PLoS One. 2020 Apr 6;15(4):e0231145. doi: 10.1371/journal.pone.0231145. eCollection 2020.
BACKGROUND & AIMS: In our previous study, a Seesaw model was proposed for the fluctuation of crucial anti- (IL-10) and pro-inflammatory (Il-6 & IL-17A) cytokines through vitamin D3. In this paper, however, it is intended to extend the mentioned model by assessing the expression mRNA levels of IL-27 and TGF-β1 as well as the changes of plasma levels of IL-27, TGF-β1, IL-17A, IL-10, and IL-6 after treatment by vitamin D3.
Venous blood samples were drawn from Healthy Participants (HP, n = 25) and First-Degree Relative Participants (FDRP, n = 25) as control groups and Multiple Sclerosis Participants (MSP, n = 25) before and after eight weeks of supplementation with 50000 IU vitamin D3. The mRNA expression and plasma concentrations were gauged by using Real-Time PCR and ELISA assay, respectively.
The mRNA surfaces of IL-27, as well as TGF-β1, were up-regulated. However, the plasma levels of TGF-β1, IL-17A, and IL-6 were significantly different among the three groups. In addition, the plasma levels of IL-27, TGF-β1, IL-10, IL-17A, and IL-6 significantly changed following the administration of vitamin D3.
The findings of this paper illustrate that anti-inflammatory cytokines could have a key role in immunomodulatory functions due to their anti-inflammatory functions. To conclude, this might contribute to preventing the pathophysiological process of MS. Also, the proposed model could be used as a preventive way on disposed people to multiple sclerosis, particularly in first degree relatives of these patients.
在我们之前的研究中，提出了一个跷跷板模型，用于解释维生素 D3 对关键抗炎（IL-10）和促炎（IL-6 和 IL-17A）细胞因子的波动。然而，在本文中，我们旨在通过评估 IL-27 和 TGF-β1 的 mRNA 表达水平以及维生素 D3 治疗后血浆中 IL-27、TGF-β1、IL-17A、IL-10 和 IL-6 的变化，来扩展上述模型。
从健康参与者（HP，n=25）和一级亲属参与者（FDRP，n=25）作为对照组和多发性硬化症参与者（MSP，n=25）中抽取静脉血样本，在补充 50000IU 维生素 D3 前后，分别通过实时 PCR 和 ELISA 测定 mRNA 表达和血浆浓度。
IL-27 和 TGF-β1 的 mRNA 表面都上调了。然而，三组之间 TGF-β1、IL-17A 和 IL-6 的血浆水平存在显著差异。此外，维生素 D3 给药后，IL-27、TGF-β1、IL-10、IL-17A 和 IL-6 的血浆水平均显著改变。
本文的研究结果表明，抗炎细胞因子由于其抗炎作用，可能在免疫调节功能中发挥关键作用。总之，这可能有助于预防 MS 的病理生理过程。此外，所提出的模型可以用作针对多发性硬化症的易患人群的预防方法，特别是在这些患者的一级亲属中。
